A 17-bp InDel (rs668420586) within goat CHCHD7 gene located in growth-related QTL affecting body measurement traits
- PDF / 1,630,239 Bytes
- 12 Pages / 595.276 x 790.866 pts Page_size
- 60 Downloads / 163 Views
ORIGINAL ARTICLE
A 17‑bp InDel (rs668420586) within goat CHCHD7 gene located in growth‑related QTL affecting body measurement traits Haixia Li1 · Xinyu Wang1 · Hong Chen1 · Lei Qu2,3 · Xianyong Lan1 Received: 14 May 2020 / Accepted: 8 September 2020 / Published online: 18 September 2020 © King Abdulaziz City for Science and Technology 2020
Abstract The Coiled-Coil-Helix-Coiled-Coil-Helix Domain Containing 7 (CHCHD7) gene was located in a growth-related major QTL that participated in the process of bone cells metabolism in animals by regulating mitochondrial copper homeostasis and cytochrome C oxidase assembly. Therefore, we speculated that CHCHD7 gene might be involved in animal growth and body size. Herein, we discovered a 17-bp insertion/deletion (indel) within the goat CHCHD7 gene. Then, we detected this variation in Shaanbei White Cashmere (SBWC, n = 1055) goats and Inner Mongolia White Cashmere (IMWC, n = 743) goats (Alathai type) using the mathematical expectation (ME) method. We then analyzed the correlation between these genotypes and goat body measurement traits. The results showed that the minor allelic frequency (MAF) was 0.011 in SBWC, and 0.048 in IMWC. In SBWC and IMWC, the reaction time by ME method was reduced by 36.78% and 27.59%, respectively, compared to the traditional method of screening samples one by one. Moreover, in SBWC goats, the 17-bp indel was significantly associated with body measurement traits (e.g. body height, and body length) in adults. In IMWC goats, the 17-bp indel was correlated with body measurement traits (e.g. body height) in weaners. In SBWC and IMWC goat populations, the body measurement traits of the individuals homozygous for 17-bp indel were higher than those in heterozygous individuals, except for the case of cannon circumference in IMWC weaners. These findings showed that the 17-bp insertion mutation within the goat CHCHD7 gene significantly affected body morphometric traits, and could provide a basis for marker-assisted selection (MAS) breeding of cashmere goats. Keywords Goat · CHCHD7 · Insertion/deletion (indel) · Mathematical expectation (ME) · Body measurement traits Abbreviations BH Body height BL Body length bp Base pair Electronic supplementary material The online version of this article (https://doi.org/10.1007/s13205-020-02434-8) contains supplementary material, which is available to authorized users. * Haixia Li [email protected] * Xianyong Lan [email protected] 1
College of Animal Science and Technology, Northwest A&F University, Yangling 712100, Shaanxi, People’s Republic of China
2
Shaanxi Provincial Engineering and Technology Research Center of Cashmere Goats, Yulin University, Yulin 719000, Shaanxi, People’s Republic of China
3
Life Science Research Center, Yulin University, Yulin 719000, Shaanxi, People’s Republic of China
BW Body weight CC Cannon circumference CcO Cytochrome C oxidase CD Chest depth CHCHD7 Coiled-coil-helix-coiled-coil-helix domain containing 7 Cu Copper CW Chest width COX23 Cytochrome C oxi
Data Loading...
