A comparative study of antimicrobial and anti-inflammatory efficiency of modified solvent evaporated and vacuum oven dri
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ORIGINAL ARTICLE
A comparative study of antimicrobial and anti-inflammatory efficiency of modified solvent evaporated and vacuum oven dried bioactive components of Pleurotus floridanus Aarti Bains1,2 • Prince Chawla3
•
Astha Tripathi1 • Pardeep Kumar Sadh4
Revised: 27 October 2020 / Accepted: 6 November 2020 Ó Association of Food Scientists & Technologists (India) 2020
Abstract In the present study, we compared vacuum microwave oven drying Vacuum Oven Drying (VOD) and modified solvent evaporation (MSE) assisted methanolic mushroom extracts for their antimicrobial and anti-inflammatory efficacy. MSE extract showed significantly (p \ 0.05) higher total phenolic content (64.4 mg/g) followed by flavonoid content (20.62 mg/g), ascorbic acid (17.54 mg/g), b-carotene content (12.52 mg/g), and lycopene (9.57 mg/g) content than that of VOD extract. MSE showed a significantly (p \ 0.05) higher zone of inhibition against all selected microorganisms as compared to VOD extract. During the time-kill study, the MSE extract inhibited significantly (p \ 0.05) higher growth of Staphylococcus aureus followed by Pseudomonas aeruginosa, Klebsiella pneumoniae, and Escherichia coli than that of VOD extract. Also, MSE extract showed significantly (p \ 0.05) higher anti-inflammatory activity in comparison
with VOD extract during the Human Red Blood Cell (HRBC) membrane stabilization test and albumin denaturation test. MSE extract revealed significantly (p \ 0.05) higher 2,2-diphenyl-1-picrylhydrazyl (DPPH) and N2O2 scavenging assay than that of VOD extract, however, statistically, MSE extract showed comparable results with Butylated Hydroxyanisole (BHA) and Butylated Hydroxytoluene (BHT). During the characterization of the selected extract, Fourier transform infrared spectroscopy confirmed the functional groups of the flavonoid content, ascorbic acid, b-carotene, and lycopene. Quantitative analysis of gallic acid (54.32 mg/g) and rutin content (14.80 mg/g) was revealed using a high-pressure liquid chromatogram.
& Prince Chawla [email protected]
Introduction
Aarti Bains [email protected] Astha Tripathi [email protected] Pardeep Kumar Sadh [email protected] 1
Shoolini University, Solan, Himachal Pradesh 173229, India
2
Department of Biotechnology, Chandigarh Group of Colleges Landran, Mohali, Punjab 140307, India
3
Department of Food Technology and Nutrition, School of Agriculture, Lovely Professional University, Phagwara, Punjab 144411, India
4
Department of Biotechnology, Chaudhary Devi Lal University, Sirsa, Haryana 125055, India
Keywords Pleurotus floridanus High-Performance Liquid Chromatography (HPLC) FTIR Antiinflammatory Methanol
Inflammation is considered as a physiological response against injury or harmful stimuli and is associated with diseases including endotoxemia and sepsis (Kamisoglu et al. 2015). In the human body, immune stimulant causes pro-inflammatory cells such as macrophages and monocytes to secrete inflammatory mediators whose uncontrolled production inside the body results in
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