A Fluorescence Study of the Interaction of Anticancer Drug Molecule Doxorubicin Hydrochloride in Pluronic P123 and F127
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ORIGINAL ARTICLE
A Fluorescence Study of the Interaction of Anticancer Drug Molecule Doxorubicin Hydrochloride in Pluronic P123 and F127 Micelles Sagar Kumar Behera 1 & Maneesha Esther Mohanty 2 & Monalisa Mohapatra 1,3 Received: 1 June 2020 / Accepted: 5 October 2020 # Springer Science+Business Media, LLC, part of Springer Nature 2020
Abstract Drug delivery systems for the sustained and target delivery of doxorubicin to tumor cells are a topic of interest due to the efficacy of the doxorubicin in cancer treatment. The use of polymers such as Pluronic is being studied widely for the formulation of doxorubicin hydrochloride. However, the basic understanding of the physicochemical properties of pluronic micelles in presence of doxorubicin hydrochloride is a very essential topic of study. Doxorubicin hydrochloride is fluorescent; this helped us to study its sensitivity towards the Pluronic microenvironment using the fluorescence technique. In this work, the interaction and place of location of doxorubicin hydrochloride in Pluronic F127 and P123 micelles has been studied extensively using steady-state fluorescence intensity, dynamic fluorescence lifetime, quenching studies, dynamic light scattering, and zeta potential measurements, at different Pluronic concentrations. Using a fluorescence quenching experiment, doxorubicin hydrochloride was found to reside near the hydrophilic PEO corona region of the Pluronic micelles. For both the Pluronic, in the concentration range of study, the micellar size was found to be below 30 nm; this may have a greater advantage for various applications. Keywords Doxorubicin hydrochloride . Pluronic, P123 . F127 . Fluorescence quenching
Introduction Anthracycline antibiotics are normally used to treat various types of cancers. These are the anti-tumor agents. Since the early 1970s these are used as effective chemotherapeutic agents [1–5]. In this category of antibiotics, Doxorubicin is one of the popular drugs employed to treat cancers of different types including, stomach, lymphoma, breast, bladder, prostate, and several others [3]. Doxorubicin is normally used in its hydrochloride salt form to enhance its solubility in the aqueous medium [6]. Doxorubicin hydrochloride (DOX) has a tetrahydroxy-anthraquinone unit, a six-member duanosamine sugar, and a dangling glycosyl moiety (Fig. 1) [7–10]. The intrinsic fluorescence of DOX acts as an essential * Monalisa Mohapatra [email protected]; [email protected] 1
Department of Chemistry, VSS University of Technology, Burla, Odisha 768018, India
2
Crop Protection Chemicals Division, CSIR-Indian Institute of Chemical Technology, Hyderabad 500007, India
3
Present address: School of Chemistry, Gangadhar Meher University, Sambalpur, Odisha 768004, India
tool in research and imaging studies such as imaging in living cells [11], membrane systems [2], human plasma [12] blood serum, and urine [13, 14]. The administration of DOX through intravenous injections have side effects associated with chemotherapeutic drugs. The systemic toxic
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