A genome-wide identification of the BLH gene family reveals BLH1 involved in cotton fiber development
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LIU et al. Journal of Cotton Research (2020) 3:26 https://doi.org/10.1186/s42397-020-00068-y
RESEARCH
Open Access
A genome-wide identification of the BLH gene family reveals BLH1 involved in cotton fiber development LIU Cuixia1,2†, LI Zhifang3†, DOU Lingling4†, YUAN Yi1, ZOU Changsong3, SHANG Haihong5, CUI Langjun1* and XIAO Guanghui1*
Abstract Background: Cotton is the world’s largest and most important source of renewable natural fiber. BEL1-like homeodomain (BLH) genes are ubiquitous in plants and have been reported to contribute to plant development. However, there is no comprehensive characterization of this gene family in cotton. In this study, 32, 16, and 18 BLH genes were identified from the G. hirsutum, G. arboreum, and G. raimondii genome, respectively. In addition, we also studied the phylogenetic relationships, chromosomal location, gene structure, and gene expression patterns of the BLH genes. Results: The results indicated that these BLH proteins were divided into seven distinct groups by phylogenetic analysis. Among them, 25 members were assigned to 15 chromosomes. Furthermore, gene structure, chromosomal location, conserved motifs, and expression level of BLH genes were investigated in G. hirsutum. Expression profiles analysis showed that four genes (GhBLH1_3, GhBLH1_4, GhBLH1_5, and GhBLH1_6) from BLH1 subfamily were highly expressed during the fiber cell elongation period. The expression levels of these genes were significantly induced by gibberellic acid and brassinosteroid, but not auxin. Exogenous application of gibberellic acid significantly enhanced GhBLH1_3, GhBLH1_4, and GhBLH1_5 transcripts. Expression levels of GhBLH1_3 and GhBLH1_4 genes were significantly increased under brassinosteroid treatment. Conclusions: The BLH gene family plays a very important role in many biological processes during plant growth and development. This study deepens our understanding of the role of the GhBLH1 gene involved in fiber development and will help us in breeding better cotton varieties in the future. Keywords: Cotton, G. hirsutum, BLH gene family, Fiber development
Introduction Cotton (Gossypium spp.) is an important crop that is used for making lint and cotton seed oil. Apart from the economic values of its fibers and seeds, cotton is also a model plant for the study of polyploids, cell elongation, * Correspondence: [email protected]; [email protected] † Liu CX, Li ZF, Dou LL contributed equally to this work. 1 Key Laboratory of the Ministry of Education for Medicinal Plant Resources and Natural Pharmaceutical Chemistry, National Engineering Laboratory for Resouce Development of Endangered Crude Drugs in the Northwest of China, College of Life Sciences, Shaanxi Normal University, Xi’an 710119, Shaanxi, China Full list of author information is available at the end of the article
and cell wall synthesis (Paterson et al. 2012). Owing to its importance, many studies on cotton have been carried out during the last decade (Li et al. 2015a; Chen et al. 2017; Gao et al. 2017; Lu et al. 2017; Zhang e
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