A large inversion in the linear chromosome of Streptomyces griseus caused by replicative transposition of a new Tn 3 fam
- PDF / 381,326 Bytes
- 8 Pages / 595.276 x 790.866 pts Page_size
- 6 Downloads / 146 Views
ORIGINAL PAPER
A large inversion in the linear chromosome of Streptomyces griseus caused by replicative transposition of a new Tn3 family transposon M. Murata • T. Uchida • Y. Yang • A. Lezhava H. Kinashi
•
Received: 5 October 2010 / Revised: 2 December 2010 / Accepted: 27 December 2010 / Published online: 15 January 2011 Springer-Verlag 2011
Abstract We have comprehensively analyzed the linear chromosomes of Streptomyces griseus mutants constructed and kept in our laboratory. During this study, macrorestriction analysis of AseI and DraI fragments of mutant 402-2 suggested a large chromosomal inversion. The junctions of chromosomal inversion were cloned and sequenced and compared with the corresponding target sequences in the parent strain 2247. Consequently, a transposon-involved mechanism was revealed. Namely, a transposon originally located at the left target site was replicatively transposed to the right target site in an inverted direction, which generated a second copy and at the same time caused a 2.5-Mb chromosomal inversion. The involved transposon named TnSGR was grouped into a new subfamily of the resolvase-encoding Tn3 family transposons based on its gene organization. At the end, terminal diversity of S. griseus chromosomes is discussed by comparing the sequences of strains 2247 and IFO13350. Keywords Streptomyces Transposon Linear chromosome Inversion Genome diversity
Communicated by Jan-Luc Pernodet. M. Murata T. Uchida Y. Yang A. Lezhava H. Kinashi (&) Department of Molecular Biotechnology, Graduate School of Advanced Sciences of Matter, Hiroshima University, 1-3-1 Kagamiyama, Higashi-Hiroshima 739-8530, Japan e-mail: [email protected] Present Address: A. Lezhava Omics Science Center, RIKEN, 1-7-22 Suehiro-cho, Tsurumi-ku, Yokohama, Kanagawa 230-0045, Japan
Introduction Streptomyces are gram-positive soil bacteria with the following interesting characteristics: (1) produce varieties of secondary metabolites including clinically useful antibiotics, (2) display one of the most complex morphological differentiation in bacteria, and (3) carry a linear chromosome of 8–10 Mb in size in place of a usual circular bacterial chromosome (Bentley et al. 2002; Ikeda et al. 2003; Ohnishi et al. 2008). Streptomyces linear chromosomes are unstable and easily cause telomere deletions spontaneously or by various mutagenic treatments; the sizes of deletions sometimes reach up to 2 Mb (Fischer et al. 1997). Mutated chromosomes show extraordinarily diverse rearrangements concomitantly with telomere deletions, such as amplification, circularization, and arm replacement (Chen et al. 2002; Fischer et al. 1998; Kinashi 2008; Qin and Cohen 2002; Volff and Altenbuchner 1998). We have been studying deletions and rearrangements of the linear chromosome of Streptomyces griseus strain 2247. The 2247 chromosome also displayed circularization (Inoue et al. 2003; Kameoka et al. 1999), arm replacement (Uchida et al. 2003), and formation of a closed racket frame structure with an unusually large palindrom
Data Loading...
