A multidisciplinary approach to study a couple of monozygotic twins discordant for the chronic fatigue syndrome: a focus
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RESEARCH
Open Access
A multidisciplinary approach to study a couple of monozygotic twins discordant for the chronic fatigue syndrome: a focus on potential salivary biomarkers Federica Ciregia1, Laura Giusti1, Ylenia Da Valle1, Elena Donadio1, Arianna Consensi2, Camillo Giacomelli2, Francesca Sernissi2, Pietro Scarpellini2, Fabrizio Maggi3, Antonio Lucacchini1* and Laura Bazzichi2
Abstract Background: Chronic Fatigue Syndrome (CFS) is a severe, systemic illness characterized by persistent, debilitating and medically unexplained fatigue. The etiology and pathophysiology of CFS remains obscure, and diagnosis is formulated through the patient’s history and exclusion of other medical causes. Thereby, the availability of biomarkers for CFS could be useful for clinical research. In the present study, we used a proteomic approach to evaluate the global changes in the salivary profile in a couple of monozygotic twins who were discordant for CFS. The aim was to evaluate differences of salivary protein expression in the CFS patient in respect to his healthy twin. Methods: Saliva samples were submitted to two-dimensional electrophoresis (2DE). The gels were stained with Sypro, and a comparison between CFS subject and the healthy one was performed by the software Progenesis Same Spot including the Analysis of variance (ANOVA test). The proteins spot found with a ≥2-fold spot quantity change and p
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