A polymeric diet rich in transforming growth factor beta 2 does not reduce inflammation in chronic 2,4,6-trinitrobenzene
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A polymeric diet rich in transforming growth factor beta 2 does not reduce inflammation in chronic 2,4,6‑trinitrobenzene sulfonic acid colitis in pre‑pubertal rats Claire Dupont‑Lucas1,2,5* , Rachel Marion‑Letellier1,5, Mathilde Pala1,5, Charlène Guerin1,5, Asma Amamou1,5, Marine Jarbeau1,5, Christine Bôle‑Feysot1,5, Lionel Nicol3,5, Amelyne David3,5, Moutaz Aziz4,5, Elodie Colasse4,5, Céline Savoye‑Collet5,6,7 and Guillaume Savoye1,5,8
Abstract Background: Pediatric Crohn’s disease is characterized by a higher incidence of complicated phenotypes. Murine models help to better understand the dynamic process of intestinal fibrosis and test therapeutic interventions. Prepubertal models are lacking. We aimed to adapt a model of chronic colitis to pre-pubertal rats and test if a polymeric diet rich in TGF-β2 could reduce TNBS-induced intestinal inflammation and fibrosis. Methods: Colitis was induced in 20 five-week-old Sprague–Dawley male rats by weekly rectal injections of increas‑ ing doses of TNBS (90 mg/kg, 140 mg/kg and 180 mg/kg) for 3 weeks, while 10 controls received phosphate-buffered saline. Rats were anesthetized using ketamine and chlorpromazine. After first administration of TNBS, 10 rats were fed exclusively MODULEN IBD® powder, while remaining rats were fed breeding chow. Colitis was assessed one week after last dose of TNBS by histopathology and magnetic resonance colonography (MRC). Results: Histological inflammation and fibrosis scores were higher in TNBS group than controls (p
