A rice GDSL esterase/lipase protein (GELP) is required for anther and pollen development
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A rice GDSL esterase/lipase protein (GELP) is required for anther and pollen development Guoqiang Yuan & Ting Zou & Xu Zhang & Miaomiao Liu & Tingting Luo & Zhiyuan He & Yang Tao & Dan Zhou & Hongfeng Zhao & Sijin Liu & Rui Liu & Menglin Zhou & Jun Zhu & Yueyang Liang & Qiming Deng & Shiquan Wang & Aiping Zheng & Aijun Wang & Huainian Liu & Lingxia Wang & Ping Li & Shuangcheng Li
Received: 31 March 2020 / Accepted: 1 September 2020 # Springer Nature B.V. 2020
Abstract Male reproductive development is vital for crop seed production. However, the mechanism underlying remains largely unknown. Here, we isolated a male sterile mutant osgelp34-1 in rice. Cytological analysis indicated that the mutant exhibited delayed degradation of anther wall layers and abnormal pollen development, which resulted in the production of shrunk and non-viable pollen grains. MutMap and CRISPR/Cas9 analysis confirmed that a point mutation in OsGELP34 is responsible for the male sterile phenotype. OsGELP34 is highly expressed in reproductive tissues and encodes a putative GDSL lipase. OsGELP34 protein is located to the endoplasmic reticulum (ER) and conserved in land plants. Collectively, our findings elaborated that OsGELP34 plays a vital role in rice male
Guoqiang Yuan and Ting Zou contributed equally to this work. Electronic supplementary material The online version of this article (https://doi.org/10.1007/s11032-020-01170-4) contains supplementary material, which is available to authorized users. G. Yuan : T. Zou : X. Zhang : M. Liu : T. Luo : Z. He : Y. Tao : D. Zhou : H. Zhao : S. Liu : R. Liu : M. Zhou : J. Zhu : Y. Liang : Q. Deng : S. Wang : A. Zheng : A. Wang : . Liu : L. Wang : P. Li : S. Li State Key Laboratory of Crop Genetic Resource Exploitation and Utilization in Southwest China, Sichuan Agricultural University, Chengdu 611130, China P. Li : S. Li (*) State Key Laboratory of Hybrid Rice, Rice Research Institute, Sichuan Agricultural University, Chengdu 611130 Sichuan, China e-mail: [email protected]
reproduction and has potential applications in rice hybrid breeding. Keywords Rice . Pollen . Anther . OsGELP34 . Male sterile
Introduction Hybrid breeding has contributed to enhancing the grain yield of rice. Male sterile lines, especially cytoplasmic male sterile (CMS) lines and environment-sensitive genic male sterile (EGMS) lines, are wildly used in hybrid seed production by providing means for eliminating the expensive and labor-intensive practice of manual emasculation. The most common cause of male sterility is pollen abortion. Pollen development occurs in anther and is initiated by the differentiation of microspore mother cells (MMCs), which undergo meiosis to form tetrads thereafter (Scott et al. 1991). Then, the microspores are released from tetrads to form the pollen wall and go through two-step mitosis to develop into mature pollen grains eventually (Scott et al. 1991). Pollen development is accompanied by the differentiation, development, and degradation of the surrounding anther somatic cell layers such as tapet
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