A simple and inexpensive method for evaluation of in vitro cell adhesion on screws
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ORIGINAL ARTICLE
A simple and inexpensive method for evaluation of in vitro cell adhesion on screws Gul I˙pek Gundogan
Received: 22 March 2020 / Accepted: 14 October 2020 Ó Springer Nature B.V. 2020
Abstract Only a limited number of techniques are available for assessing the effect of different coating materials on cell adherence to screws. In this study, we describe a simple and inexpensive method for evaluation of cell adhesion on irregular surfaces such as the surgical or implant screws. For this purpose, we prepared semi-submerged screws in the petri dishes using agar. Using BSA- or HA-coated screws, we tested whether BSA or HA could improve cell adherence when used as coating materials. Agarcoated screws were used as internal control. Then the ‘‘ratio of cell adherence’’ was calculated by subtracting the reference RCA value obtained from the agar coated screws (internal control). When compared to that of the non-coated screws both the HA- and BSAcoating improved cell adherence on the screws by 2.34 and 2.72 fold respectively. Similarly, MTT assay data revealed that the metabolic capacities of cells on HAor BSA-coated screws were improved by 2.36 and 2.86 fold respectively. These findings suggest that this protocol can be used for comparing the ability of cells to attach on irregular surfaces such as dental or orthopedic screws and assessing their viability.
G. I˙. Gundogan (&) Department of Histology and Embryology, Faculty of Medicine, Istanbul Yeni Yuzyil University, Maltepe Mahallesi, Yılanlı Ayazma Caddesi, No: 26, Cevizlibag˘, Zeytinburnu, 34010 I˙stanbul, Turkey e-mail: [email protected]
Keywords Irregular surfaces Dental Orthopedics Cell culture
Introduction Implants are used in various clinical applications including the areas of orthopedics or dentistry. Studies in implant technology have expanded significantly over the last decades. Cellular adhesion is essential for the successful integration of implants into the bone tissue. Therefore, biocompatible surfaces that promote a favorable environment both fort the cells and host tissues are necessary for dental implants to successfully modulate the adhesion, proliferation and phenotypic expression of osteoblastic cells (Osman and Swain 2015). Several different experimental approaches exist for evaluation of cell adhesion efficiency. For example, the ‘‘wash off’’ technique is a simple method for the determination of short term adherent cells where the number of the detached cells from the implant surfaces can be counted (Garcı´a and Gallant 2003). Additionally, as reviewed by Khalili and Ahmad (2015), several different methods such as the cytodetachment technique, micropipette aspiration technique, force spectroscopy measurement methods, biomembrane force probe, optical tweezers, spinning disc methods, etc. can also be employed (Khalili and Ahmad 2015).
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Cytotechnology
However, these techniques are not easily accessible in every laboratory and they may not allow for an effe
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