A smart DNA nanodevice for ATP-activatable bioimaging and photodynamic therapy
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tps://doi.org/10.1007/s11426-020-9764-9
SPECIAL ISSUE: 2020 Emerging Investigator Issue
A smart DNA nanodevice for ATP-activatable bioimaging and photodynamic therapy 1,2†
Bei Liu
2†
2,3
2,3,4
, Rui Ma , Jian Zhao , Yuliang Zhao
2,3,4*
& Lele Li
1
2
School of Science, Minzu University of China, Beijing 100081, China; CAS Key Laboratory for Biomedical Effects of Nanomaterials and Nanosafety and CAS Center for Excellence in Nanoscience, National Center for Nanoscience and Technology, Beijing 100190, China; 3 College of Materials Science and Optoelectronic Technology, University of Chinese Academy of Sciences, Beijing 100049, China; 4 GBA Research Innovation Institute for Nanotechnology, Guangdong 510700, China
Received March 4, 2020; accepted April 29, 2020; published online June 5, 2020
Rational design of activatable photosensitizers for controlled generation of singlet oxygen remains a challenge for precise photodynamic therapy (PDT). Herein, we present an aptamer-based nanodevice for adenosine 5′-triphosphate (ATP)-activatable bioimaging and PDT. The nanodevice is constructed by modifying ATP-responsive duplex DNA units and polyethylene glycol on the surface of a gold nanoparticle (AuNP) through the thiolate-Au chemistry. The DNA units were designed by the hybridization of the ATP aptamer strand with a methylene blue (MB)-modified complementary DNA (cDNA). The close proximity of MB to the surface of AuNP results in the low photodynamic activity of MB (OFF state). Once internalized into cancer cells, the ATP-binding induced conformation switch of aptamer strand leads to the release of the MB-bearing DNA strand from AuNPs, resulting in the activatable generation of singlet oxygen under light irradiation (ON state). We demonstrate that the DNA nanodevice represents a promising platform for ATP-responsive bioimaging and specific PDT in vitro and in vivo. This work highlights a potential way for specific tumor diagnosis and therapy. DNA nanotechnology, ATP-activatable, photodynamic therapy, bioimaging Citation:
Liu B, Ma R, Zhao J, Zhao Y, Li L. A smart DNA nanodevice for ATP-activatable bioimaging and photodynamic therapy. Sci China Chem, 2020, 63, https://doi.org/10.1007/s11426-020-9764-9
1 Introduction As a noninvasive medical technique, photodynamic therapy (PDT) has emerged as a reliable cancer treatment modality [1–6]. PDT relies on the generation of cytotoxic singlet oxygen upon light irradiation of photosensitizers (PSs). Compared to conventional chemotherapy, PDT provides additional tissue selectivity because of the pre-determined PSs accumulation and light placement [7–14]. However, the “alwaysactive” model and low selectivity of the currently used PSs is
†These authors contributed equally to this work. *Corresponding author (email: [email protected])
a formidable challenge for the clinical application of PDT [15,16]. Recently, activatable PSs have been developed as smart PSs to minimize the nonspecific side effects [17–19]. These PSs can be selectively turned on by appropriate triggers, allow
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