A study of the mechanism of lncRNA-CR594175 in regulating proliferation and invasion of hepatocellular carcinoma cells i
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RESEARCH ARTICLE
Open Access
A study of the mechanism of lncRNACR594175 in regulating proliferation and invasion of hepatocellular carcinoma cells in vivo and in vitro Quan Liu1†, Xuxu Yu2†, Minjie Yang2, Xiangke Li2, Xuejia Zhai2, Yujin Lian2, Zhong Chen2, Qingxia Fan2, Lijie Song2* and Wencai Li3*
Abstract Background: Hepatocellular carcinoma (HCC) is one of the cancers of highest incidence and mortality worldwide. The proliferation and invasion of tumor cells are the main reason for poor prognosis after HCC surgery. Long noncoding RNA (lncRNA) has been shown to play a key role in the progression of HCC. LncRNA-CR594175 is one of the highly expressed lncRNAs in HCC tumors and their metastatic tumors that we have obtained by the Highthroughput screening method. Methods: To elucidate the role of lncRNA-CR594175 in regulating the proliferation and invasion of human hepatoma cell line, HepG2, we operated through lncRNA-CR594175 silencing to inhibit the progression of HCC, either through in vitro or in vivo experiments. Results: We found that lncRNA-CR594175 was lower in adjacent non-cancerous tissues than in primary HCC, and was lower in primary HCC than in its metastasis. Silencing of lncRNA-CR594175 inhibited the proliferation and invasion of HepG2 cells and growth of subcutaneous tumors. The results revealed that lncRNA-CR594175, as a RNA sponge, broke the negative regulation of hsa-miR-142-3p on Catenin, beta-1 (CTNNB1), and once lncRNA-CR594175 was silenced, the hsa-miR142-3p regained its negative regulation on CTNNB1 which can promote HCC progression by activating the wnt pathway. Conclusions: Our present study demonstrated for the first time that lncRNA-CR594175 silencing suppressed proliferation and invasion of HCC cells in vivo and in vitro by restoring the negative regulation of hsa-miR-142-3p on CTNNB1, laying a solid theoretical base for using lncRNA-CR594175 as genetic target therapy for HCC and offering a reasonable explanation for inactivation of miRNA in different tumors or in the tumor at different stages. Keywords: lncRNA, lncRNA-CR594175, Hepatocellular carcinoma, Hsa-miR142-3p, HepG2
* Correspondence: [email protected]; [email protected] † Quan Liu and Xuxu Yu contributed equally to this work and are co-first authors. 2 Department of Oncology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, China 3 Department of Pathology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, China Full list of author information is available at the end of the article © The Author(s). 2020 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indi
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