A Turn-on Biosensor-Based Aptamer-Mediated Carbon Quantum Dots Nanoaggregate for Acetamiprid Detection in Complex Sample
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A Turn-on Biosensor-Based Aptamer-Mediated Carbon Quantum Dots Nanoaggregate for Acetamiprid Detection in Complex Samples Zhe Jiao 1 & Hongfeng Zhang 2 & Shaohe Jiao 3 & Zongning Guo 4 & Dan Zhu 1 & Xiaofang Zhao 5 Received: 2 August 2018 / Accepted: 31 October 2018 / Published online: 15 November 2018 # Springer Science+Business Media, LLC, part of Springer Nature 2018
Abstract In this paper, we developed a new biosensor-based on aptamer-mediated carbon quantum dots (apt-CQDs) nanoaggregate for acetamiprid detection. The acetamiprid aptamer, 5′- and its complementary DNA were labeled on CQDs, respectively. The hybridization of aptamer and its complementary DNA triggered the formation of nanoaggregate of CQDs, leading to the fluorescence quenching of the CQDs. Upon addition of acetamiprid, specific recognition of acetamiprid caused the release of the cDNA-CQDs and the fluorescence intensity recovered which was linearly related to the concentration of acetamiprid. The sensor provided a linear range from 0.2 to 20 ng/L for acetamiprid with a detection limit of 0.04 ng/L in an aqueous buffer. The same linear range was obtained in spiked human serum samples and lettuce samples with similar detection limits (0.15 and 0.11 ng/L), demonstrating high robustness of the sensor in a complex sample matrix. The as-prepared biosensor was used to monitor acetamiprid level in real samples with recovery ranged from 86 to 107%, and RSDs ranged from 1.3–6.9% (n = 3). The proposed study not only provided a simple method through CQDs nanoaggregate and also provided a turn-on detection mode for acetamiprid in real samples with high signal-to-noise ratios.
Keywords Carbon quantum dots . Aptamer . Biosensor . Acetamiprid
Introduction Zhe Jiao and Hongfeng Zhang contributed equally to this work. Electronic supplementary material The online version of this article (https://doi.org/10.1007/s12161-018-1393-9) contains supplementary material, which is available to authorized users. * Zhe Jiao [email protected] Xiaofang Zhao [email protected] 1
School of Environment and Civil Engineering, Dongguan University of Technology, Dongguan 523808, China
2
Guangzhou Center for Disease Control and Prevention, Guangzhou 510440, China
3
Tobacco Science Institute of Jiangxi Province, Nanchang 330025, China
4
Integrated Technology Center of Dongguan Entry-Exit Inspection and Quarantine Bureau, Dongguan 523073, China
5
School of Electrical Engineering and Intelligentization, Dongguan University of Technology, Dongguan 523808, China
Aptamers are artificial functional oligonucleic acids selected in vitro through systematic evolution of ligands by exponential enrichment (SELEX) from random-sequence nucleic acids libraries (Ellington and Szostak 1992). Like antibodies, they exhibited high specificity and affinity for a wide range of target molecules, such as protein, small molecules, amino acids and even cells. Besides, aptamer possesses a variety of merits over antibodies such as smaller size, easier artificial synthesis, modification and better stabil
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