Amplified electrochemical determination of UO 2 2+ based on the cleavage of the DNAzyme and DNA-modified gold nanopartic
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ORIGINAL PAPER
Amplified electrochemical determination of UO22+ based on the cleavage of the DNAzyme and DNA-modified gold nanoparticle network structure Chen Cao 1 & Jinquan Liu 2,3,4 & Shuangyang Tang 5 & Zhongran Dai 6 & Fubing Xiao 2,3 & Weiqing Rang 2 & Ling Liu 2 & Tuo Chen 2 & Yali Yuan 1 & Le Li 2,3,6 Received: 31 October 2019 / Accepted: 3 April 2020 # Springer-Verlag GmbH Austria, part of Springer Nature 2020
Abstract A superior electrochemical biosensor was designed for the determination of UO22+ in aqueous solution by integration of DNAzyme and DNA-modified gold nanoparticle (DNA-AuNP) network structure. Key features of this method include UO22+ inducing the cleavage of the DNAzyme and signal amplification of DNA-AuNP network structure. In this electrochemical method, the DNA-AuNP network structure can be effectively modified on the surface of gold electrode and then employed as an ideal signal amplification unit to generate amplified electrochemical response by inserting a large amount of electrochemically active indicator methylene blue (MB). In the presence of UO22+, the specific sites on DNA-AuNP network structure can be cleaved by UO22+, releasing the DNA-AuNP network structure with detectable reduction of electrochemical response intensity. The electrochemical response intensity is related to the concentration of UO22+. The logarithm of electrochemical response intensity and UO22+ concentration showed a wide linear range of 10~100 pM, and the detection limit reached 8.1 pM (S/N = 3). This method is successfully used for determination of UO22+ in water samples. Keywords AuNPs . Electrochemical . Biosensor . UO22+ . Methylene blue . DNAzyme . Signal amplification . DNA-AuNP network . DPV . Water sample
Introduction Uranium, a very important radioactive element, is widely distributed in the environment, such as the atmosphere, soil, or water [1]. In the past half century, uranium has been widely used in nuclear research, nuclear fuel, nuclear weapons, and
other fields, so its demand has been gradually increased. Uranium mining, uranium enrichment, and spent fuel postnuclear industrial processes are accompanied by the generation and release of a large amount of uranium-containing wastewater [2]. Uranium has both high chemotoxicity and radiotoxicity. Therefore, uranium contamination has posed a
Electronic supplementary material The online version of this article (https://doi.org/10.1007/s00604-020-04263-1) contains supplementary material, which is available to authorized users. * Yali Yuan [email protected] * Le Li [email protected] 1
School of Chemistry and Chemical Engineering, University of South China, Hengyang 421001, Hunan, People’s Republic of China
2
School of Public Health, University of South China, Hengyang 421001, Hunan, People’s Republic of China
3
Key Laboratory of Health Hazard Factors Inspection and Quarantine, University of South China, Hengyang 421001, People’s Republic of China
4
State Key Laboratory of Chemo/Biosensing and Chemometrics, Hunan University, Changsha410082,
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