Analysis of ADAM9 regulation and function in vestibular schwannoma primary cells
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BMC Research Notes Open Access
RESEARCH NOTE
Analysis of ADAM9 regulation and function in vestibular schwannoma primary cells Anja Nattmann1†, Maria Breun1*†, Camelia M. Monoranu2, Cordula Matthies1, Ralf‑Ingo Ernestus1, Mario Löhr1 and Carsten Hagemann1
Abstract Objective: Recently, we described a disintegrin and metalloproteinase 9 (ADAM9) overexpression by Schwann cells of vestibular schwannoma (VS) and suggested that it might be a marker for VS tumor growth and invasiveness. This research note provides additional data utilizing a small cohort of VS primary cultures and tissue samples. We exam‑ ined whether reconstitution of Merlin expression in VS cells regulates ADAM9 protein expression and performed lenti‑ viral ADAM9 knock down to investigate possible effects on VS cells numbers. Moreover, the co-localization of ADAM9 and Integrins α6 and α2β1, respectively, was examined by immunofluorescence double staining. Results: ADAM9 expression was not regulated by Merlin in VS. However, ADAM9 knock down led to 58% reduction in cell numbers in VS primary cell cultures (p
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