Analysis of clinical important of LncRNA-HOTAIR gene variations and ovarian cancer susceptibility
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ORIGINAL ARTICLE
Analysis of clinical important of LncRNA‑HOTAIR gene variations and ovarian cancer susceptibility Narmella Saeedi1 · Saeid Ghorbian1 Received: 23 July 2020 / Accepted: 29 August 2020 © Springer Nature B.V. 2020
Abstract LncRNAs are of functional long non-coding RNAs, which have been shown to be involved in critical pathways in cancer development. LncRNA-HOTAIR gene overexpression has been reported in several cancers. The aim of this study was to evaluate the associations between two variants of lncRNA-HOTAIR (rs1899663 G>T and rs4759314 A>G) gene polymorphisms and the risk of ovarian cancer (OC) susceptibility. We performed a case and control analysis on two hundred individuals consisting of 100 cases with OC and 100 women cancer-free in East Azerbaijan of Iranian population. To evaluate the association between two SNPs of lncRNA-HOTAIR with the risk of OC susceptibility used the polymerase chain reactionrestriction fragment-length polymorphism (PCR-RFLP) method. We revealed that two SNPs in the lncRNA-HOTAIR gene were significantly associated with the risk of OC. The dominant model of rs4759314 in lncRNA-HOTAIR (AA vs. GA/GG) showed a significantly increased risk with an OR of 10.036 (CI 2.253–44.712, P = 0.000); the recessive model of rs1899663 (TT vs. GT/GG) revealed a significantly increased risk with OR of 0.910 (CI 0.856–0.968; P = 0.002). In addition, our findings demonstrated that the 4759314G (OR 13.500; CI 3.146–57.940; P = 0.000) and 1899663T (OR 3.273; CI 1.433–7.475; P = 0.003) alleles are increased the risk of OC susceptibility. Our findings provide evidence that the specific genetic variants in lncRNA-HOTAIR gene may affect OC susceptibility in an Iranian population. Keywords Ovarian cancer · lncRNA-HOTAIR · HOTAIR · Ovarian susceptibility
Introduction Ovarian cancer (OC) is one of the popular gynecological malignancies, which records for a bigger mortality amount and the basic molecular pathway is even unrecognized [1]. The prevalence of OC represented roughly four percent of tumor cases in women, which ordinarily correlated with asymptomatic in the initial stages [2]. To date, a controversy of the radiotherapy and chemotherapy developed in cancer therapy, but the 5-year survival rate has remained around Lowe [3]. There are several risk factors related to OC such as age, obesity, family history, and other related Electronic supplementary material The online version of this article (https://doi.org/10.1007/s11033-020-05797-6) contains supplementary material, which is available to authorized users. * Saeid Ghorbian [email protected]; s_ghorbian@iau‑ahar.ac.ir 1
Department of Molecular Genetics, Ahar Branch, Islamic Azad University, Ahar, Iran
candidate genes [4]. Almost ten percent of invasive OC developed from genetic susceptibility [5]. One of these predisposing genetic biomarkers suggested that the long non-coding RNAs (lncRNAs). The findings of molecular profiling approaches in diverse cancerous tissues have been revealed that the gene expression levels of lncRNAs
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