Analysis of Human Spleen Contamination
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1063-OO09-13
Analysis of Human Spleen Contamination Martin Kopani1, Martin Weis2, Julius Dekan3, Jan Jakubovsky1, and Marcel Miglierini3 1 Department of Pathology, Comenius University, School of Medicine, Sasinkova 4, Bratislava, 811 08, Slovakia 2 Department of Physics, Slovak University of Technology, Faculty of Electrical Engineering and Information Technology, Ilkovicova 3, Bratislava, 81219, Slovakia 3 Department of Nuclear Physics, Slovak University of Technology, Faculty of Electrical Engineering and Information Technology, Bratislava, 81219, Slovakia ABSTRACT We identified both crystalline and amorphous phase of human spleen particles. The silicon particles in the spleen were 10-30 μm large. Silicon, silicon-aluminium and silicon-calcium particles by EDX were found. We assume that the silicon must enter the organism from the external environment and the presence of silicon in the human spleen is consequence of cleaning function of the human spleen. The Mössbauer spectroscopy of studied tissues revealed different phase of iron oxide in the human spleen. On the ground of this consideration we may claim that all samples of investigated tissues exhibit presence of two different paramagnetic iron phases, both based on three-valent (Fe3+) atoms. Consequently, only 4 different iron-oxides correspond to the experimental results. Multielemental composition of iron particles was found by EDX analysis. We suppose that pH and time are significant factors influencing biomineralization of iron in the human spleen. INTRODUCTION Besides carbon, oxygen, and nitrogen, numerous other elements and their compounds are significant in the body of humans and other animals. Some elements get into the internal environment of living bodies, accumulate in various organs, and disturb the systems that participate in elimination thereof; or on contrary, they accumulate in the bodies because of congenital or acquired metabolic disturbances. Iron can be found in human body mainly in the form of ferritin. This protein creates spherical formation in size of 12 nm. The size of the core of ferritin is 8 nm and consists of ferrihydrite - 5Fe2O3.9H2O with various amount of phosphorus. The role of polypeptide coat of ferritin (Ft-H form) is to catalyze Fe2+ ions to Fe3+ions. Ft-H form of polypeptide coat helps to mineralize the iron. Studies performed via nanodiffraction showed that physiological ferritin consists from crystalline ferrihydrite and amorphous iron hydroxide [1]. In the core of pathological ferritin, wüstite (FeO) and magnetite–like structure prevailed. In excessive volume of iron in the organism, the iron is stored in cells in the form of hemosiderin [2]. Hemosiderin is considered to be a proteolytic product of ferritin [3]. Besides differences in the diffraction image between the samples of hemosiderin, some differences are also found in their element composition [4,5]. The 57Fe Mössbauer spectroscopy enables to determine the composition of iron compound in the sample. Position, number, and intensity of absorbed radiation give us
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