Animal Cell Technology: Basic & Applied Aspects Proceedings of t

Animal cell technology is a growing discipline of cell biology which aims not only to understand structures, functions and behaviors of differentiated animal cells but also to uncover their abilities for industrial and medical purposes. The goal of animal

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JAACT'96 YOKOHAMA JAACT'96 ORGANIZING COMMITTEE Chairperson:

KazuoNagai

(Department of Bioengineering, Tokyo Institute of Technology)

Secretary General:

Masaaki Wachi

(Department of Bioengineering, Tokyo Institute of Technology)

Organizing Boards:

Akio Ametani Shuichi Hashizume Kazuki Shin ohara

(The University of Tokyo) (Morinaga Institute of Biological Science) (National Research Institute of Fisheries Science) (The University of Tokyo) (Kirin Brewery Co., Ltd.)

Eiji Suzuki Takamoto Suzuki

Program Committee Masuo Aizawa Toshihiro Akaike John Barford Shun'ichi Dosako Hiroyoshi Hoshi Wei-ShoulIn Koji Ikura David Barnes David Jayme Shuichi Kaminogawa Kin'ichi Kawamura Yasuo Kitagawa Yoji Mitsui Alan Moore Tadao Ohno Ralf Portner Ryuzo Sasaki Kazuo Shin Hajime Unno John Werenne

Tokyo Institute of Technology Tokyo Institute of Technology University of Sydney Snow Brand Milk Products Co.,Ltd. Research Institute of Functional Peptides University of Minnesota Kyoto Institute of Technology Oregon State University Life Technologies Inc. The University of Tokyo Komatsugawa Chemical Engineering Co.,Ltd. Nagoya University Nat. Inst. Biosci. Human-Tech. Microbiological Associates Inc. Riken Gene Bank Technische Universitat Hamburg-Harburg Kyoto University Meiji Milk Products Co.,Ltd. Tokyo Institute of Technology Universite Libre de Bruxelles

Animal Cell Technology: Basic & Applied Aspects Volume 9 Proceedings of the Ninth Annual Meeting of the Japanese Association for Animal Cell Technology, Yokohama, Japan, September 1-4, 1996

Edited by

K. NAGAI Department of Bioengineering, Tokyo Institute of Technology, Yokohama, Japan

and

M. WACHI Department of Bioengineering, Tokyo Institute of Technology, Yokohama, Japan

SPRINGER SCIENCE+BUSINESS MEDIA, B.V.

A C.I.P. Catalogue record for this book is available from the Library of Congress.

ISBN 978-94-010-6170-4 ISBN 978-94-011-5161-0 (eBook) DOl 10.1007/978-94-011-5161-0

Printed on acid-free paper

All Rights Reserved © 1998 Springer Science+Business Media Dordrecht Originally published by Kluwer Academic Publishers in 1998 Softcover reprint of the hardcover lst edition 1998 No part of the material protected by this copyright notice may be reproduced or utilized in any form or by any means, electronic or mechanical, including photocopying, recording or by any information storage and retrieval system, without written permission frdm the copyright owner.

Contents Preface

xi

I. Establishing new cell lines and characterizing molecular biological properties of cultured cells Stress-inducible gene expression in fish cells M. Yamashita

1

Cell culture and some properties of fish immunocytes T. Watanabe, and T. Moritomo

5

Telomerase regulation in differentiated serum-free mouse embryo cells Y. Katakura, K. Yamamoto, O. Miyake, E. Nakata, T. Yasuda, N. Uehara, D. W. Barnes, and S. Shirahata

11

Immortalization of mammalian central nervous system stem cells from p53 knock-out forebrain T. Hisatsune, K. Yamada, R. D. G. McKay, and S. Kaminogawa

17

Neuroprotective