Antibacterial mechanism of brevilaterin B: an amphiphilic lipopeptide targeting the membrane of Listeria monocytogenes
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BIOTECHNOLOGICALLY RELEVANT ENZYMES AND PROTEINS
Antibacterial mechanism of brevilaterin B: an amphiphilic lipopeptide targeting the membrane of Listeria monocytogenes Yangliu Liu 1 & Aijin Ma 1 & Panpan Han 1 & Zhou Chen 1 & Yingmin Jia 1 Received: 23 July 2020 / Revised: 20 October 2020 / Accepted: 31 October 2020 # Springer-Verlag GmbH Germany, part of Springer Nature 2020
Abstract Antimicrobial peptides (AMPs) are recognized as promising safe alternatives to antibiotics for its low drug-resistance. Brevilaterin B, a newly discovered antimicrobial lipopeptide produced by Brevibacillus laterosporus S62-9, exhibits efficient antibacterial activity on Listeria monocytogenes with a minimum inhibitory concentration of 1 μg mL−1. The present research aimed to investigate the antibacterial mechanism of brevilaterin B against Listeria monocytogenes. Brevilaterin B caused membrane depolarization and the breakup of the cytomembrane as measured by 3,3-dipropylthiadicarbocyanine iodide and transmission electron microscopy, respectively. Using 1,2-dipalmitoyl-sn-glycero-3-phosphocholine and 1,2-dipalmitoyl-snglycero-3-phospho-rac-(1-glycerol) sodium salt (7:3) as a model membrane, results proved that brevilaterin B could bind to liposomes, integrate into the lipid bilayer, and consequently increase the permeability of liposomes to calcein. The secondary structure of brevilaterin B also changed from an unstructured coil to a mainly β-sheet conformation as measured by circular dichroism. Brevilaterin B exhibits antibacterial activity by a membrane interaction mechanism, which provides a theoretical basis for using brevilaterin B as a promising natural and effective antimicrobial agent against pathogenic bacteria. Key points • Brevilaterin B exhibited antibacterial activity against Listeria monocytogenes. • Brevilaterin B exhibited membrane interaction mechanism. • Brevilaterin B showed conformational change when interacted with liposome. Keywords Antimicrobial peptides . Brevibacillus laterosporus S62-9 . Brevilaterin B . Antibacterial mechanism . Listeria monocytogenes . Peptide-lipid interaction
Introduction Pathogenic microorganisms can cause serious infections that threaten human health and can cause great economic losses in agriculture. The abuse of antibiotics has resulted in an increase in drug-resistant bacteria, which presents a challenge for providing global environmental safety. Therefore, there is an urgent need to develop novel antimicrobial agents. As one of the most promising new antimicrobial agents, antimicrobial
* Yingmin Jia [email protected] 1
School of Food and Health, Beijing Technology and Business University, No.33 Fucheng Road, Haidian District, Beijing 100048, China
peptides (AMPs) have received extensive attention (Shin et al. 2016; Wang et al. 2019). Brevibacillus spp., a species of important bio-control bacteria, produce AMPs that efficiently inhibit bacteria, fungi, and even cancer cells (Yang and Yousef 2018). These species are regarded as a rich source of AMPs, and to date more than 3
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