Antibody Engineering
Antibodies are indispensable tools for research, diagnosis, and therapy. Recombinant approaches allow the modification and improvement of nearly all antibody properties, such as affinity, valency, specificity, stability, serum half-life, effector function
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Roland Kontermann
l
Stefan Du¨bel
Editors
Antibody Engineering Volume 1 Second Edition
Editors Prof. Dr. Roland Kontermann (Biomedical Engineering) Institut fu¨r Zellbiologie und Immunologie Universita¨t Stuttgart Allmandring 31 70569 Stuttgart Germany [email protected]
Professor Dr. Stefan Du¨bel Technische Universita¨t Braunschweig Institut fu¨r Biochemie und Biotechnologie Spielmannstraße 7 38106 Braunschweig Germany [email protected]
ISBN 978-3-642-01143-6 e-ISBN 978-3-642-01144-3 DOI 10.1007/978-3-642-01144-3 Springer Heidelberg Dordrecht London New York Library of Congress Control Number: 2010922366 # Springer-Verlag Berlin Heidelberg 2001, 2010 Originally published in one volume within the series Springer Lab Manuals This work is subject to copyright. All rights are reserved, whether the whole or part of the material is concerned, specifically the rights of translation, reprinting, reuse of illustrations, recitation, broadcasting, reproduction on microfilm or in any other way, and storage in data banks. Duplication of this publication or parts thereof is permitted only under the provisions of the German Copyright Law of September 9, 1965, in its current version, and permission for use must always be obtained from Springer. Violations are liable to prosecution under the German Copyright Law. The use of general descriptive names, registered names, trademarks, etc. in this publication does not imply, even in the absence of a specific statement, that such names are exempt from the relevant protective laws and regulations and therefore free for general use. Cover design: deblik Berlin, Germany Printed on acid-free paper Springer is part of Springer Science+Business Media (www.springer.com)
Foreword
Antibodies, naturally produced for protection by a variety of organisms, are also extremely powerful tools for research, diagnosis, and therapy. Since publication of the first edition of Antibody Engineering in 2001, the field of antibody research and development (R&D) has continued to grow at a remarkable pace. The research arena has seen advances in understanding structure-function relationships, antibody engineering techniques, and production of various antibody fragments. Clinical development has expanded, with novel monoclonal antibodies directed toward an array of targets entering the study at a rapid pace and the study of more than 200 monoclonal antibodies as treatments for a wide variety of diseases on‐going. A key feature of the global surge in antibody R&D activity is the need for updated information by both novice and experienced researchers. The publication of this second edition of Antibody Engineering is thus timely. In this manual, Roland Kontermann and Stefan Du¨bel provide comprehensive coverage of both new and well-established techniques. Volume 1 reviews techniques that serve as the foundation for antibody research (e.g. humanization, antibody production in eukaryotic expression systems), key information on the measurement of antibody structure and function, and curr
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