Application of the SureTect Detection Methods for Listeria monocytogenes and Listeria spp. in Meat, Dairy, Fish, and Veg
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Application of the SureTect Detection Methods for Listeria monocytogenes and Listeria spp. in Meat, Dairy, Fish, and Vegetable Products David Rodriguez-Lazaro & Patricia Gonzalez-García & Antonio Valero & Marta Hernandez
Received: 9 August 2014 / Accepted: 9 August 2014 / Published online: 20 August 2014 # Springer Science+Business Media New York 2014
Abstract The aim of this study was to evaluate the application of the Listeria monocytogenes and Listeria spp. SureTect detection methods for a rapid and sensitive detection in an ample range of food products: raw pork and poultry meat, Spanish chorizo, pate, smoked salmon, raw sheep milk cured cheese, and ready-to-eat lettuce salad. The combination of a 24-h enrichment in the 24 Listeria Enrichment Broth (LEB) coupled to a rapid bacterial DNA extraction and real-time polymerase chain reaction (RTi-PCR) using the specific SureTect methods detected down to 2–6 L. monocytogenes CFU per sample in less than 27 h on the food categories tested. Furthermore, the applicability of L. monocytogenes and Listeria spp. SureTect detection methods in real samples was assessed using 303 food samples, obtaining at least the same analytical performance as the international reference method ISO 11290-1. Keywords Real-time PCR . Detection . Listeria monocytogenes . Food
Introduction Listeria monocytogenes is widely distributed in the environment and represents a major threat in food safety due to its D. Rodriguez-Lazaro (*) : P. Gonzalez-García : M. Hernandez Instituto Tecnológico Agrario de Castilla y León (ITACyL), Carretera de Burgos Km 119, 47009 Valladolid, Spain e-mail: [email protected] D. Rodriguez-Lazaro Microbiology Section, Faculty of Sciences, University of Burgos, Plaza Misael Bauñuelos s/n, 9001 Burgos, Spain A. Valero Department of Food Science and Technology, University of Cordoba, Campus de Rabanales, Edificio Darwin, 14014 Córdoba, Spain
unique capacity to survive and propagate in the foodprocessing environments and a large variety of foods (Gandhi and Chikindas 2007; Sauders and Wiedmann 2007; Todd and Notermans 2011). L. monocytogenes has been involved in outbreaks associated with a wide spectrum of processed foods (Carpentier and Cerf 2011; Todd and Notermans 2011), but it is most often associated with ready-to-eat food products that are consumed without prior cooking (Norton and Braden 2007). Food safety regulations have tended to adopt a zero tolerance attitude for L. monocytogenes in these products (Rodriguez-Lazaro et al. 2014). Conventional methods for the detection of L. monocytogenes in food involve two consecutive growth steps and a final isolation step in specific solid media and a subsequent final identification of the presumptive colonies by biochemical and serological tests (Rodríguez-Lazaro and Hernandez 2014). It is labor-intensive and time-consuming, being needed up to 7 days for a final positive result (RodríguezLazaro and Hernandez 2014). As a consequence, culture standard methods do not prove an effective quick solution to tackle the food chain n
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