Application of Upconversion Luminescence of NaYF 4 :Yb,Er Nanoparticles to Study Protein Coagulation Dynamics
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Application of Upconversion Luminescence of NaYF4:Yb,Er Nanoparticles to Study Protein Coagulation Dynamics A. A. Skaptsova, *, S. O. Ustalkova, A. H. M. Mohammeda, A. M. Zakharevicha, A. A. Kozyrevb, E. A. Sagaidachnayaa, and V. I. Kochubeya,c a Saratov
b
State University, Saratov, 410012 Russia National Research Nuclear University “MEPhI,” Moscow, 115409 Russia c National Research Tomsk State University, Tomsk, 634050 Russia *e-mail: [email protected]
Received December 10, 2019; revised February 7, 2020; accepted February 28, 2020
Abstract—We present a method for measuring the dynamics of the internal temperature of biological tissues and the thickness of a denatured layer during laser thermolysis. This method is based on measuring the intensities of three luminescence bands of NaYF4:Yb,Er upconversion nanoparticles in the green and red parts of the visible spectrum. Ovalbumin was used as a model biological tissue, the general denaturation patterns of ovalbumin were also studied using plasmonic photothermal therapy. Keywords: ovalbumin, abnormal temperature increase, gold, nanothermometry, plasmonic photothermal therapy, luminescence DOI: 10.1134/S0030400X20070218
1. INTRODUCTION Denaturation of biological tissues is a key task of many branches of medicine, such as nanosurgery [1], oncology [2], and cosmetology [3]. Modern nanotechnology, developing new approaches and methods for the application of nanoscale structures, makes it possible to conduct operations at the microscale level. Many approaches have been developed, for example, plasmonic photothermal therapy (PPTT) using gold nanostructures to heat them inside biological tissues [4] and the use of nanocapsules for delivery drugs causing tissue necrosis [5], iron oxides heated by a variable magnetic field inside biological tissue [6, 7], or carbon nanotubes [8]. All these approaches are based on the delivery of a given amount of heat to a specific area of biological tissue with the goal of thermal destruction of cells [9]. However, methods for monitoring and controlling the heat delivery process are not sufficiently developed to be applied in clinical practice. The development of control and management methods is a main task for the successful application of thermal destruction of biological tissues. Recent studies have shown that the presence of several survived cancer cells under the denatured tissue crust leads to further intensive tumor development [10]. Currently, various methods for determining the temperature inside biological tissue are applied, such as magnetic resonance imaging (MRI) [11], acoustic imaging [12], and nanothermometry [13]. Determin-
ing the temperature of the nanoparticles themselves, rather than the environment, is a difficult task [14]; it can be achieved by Raman scattering [15] and nanothermometry. Nanothermometry is one of the promising approaches for determining the temperature inside biological tissues, based on the application of the dependence of the luminescence spectra of semiconductor nanoparticles on the am
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