Assessment of Mitochondrial Stress in Neurons: Proximity Ligation Assays to Detect Recruitment of Stress-Responsive Prot
Mitochondria are highly integrated organelles that must readily alter organelle physiology to adapt to the changing environment of neurons. Failure in the mechanisms regulating organelle adaptation and homeostasis manifests as perturbations in bioenergeti
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Michael Aschner Lucio Costa Editors
Cell Culture Techniques Second Edition
Neuromethods
Series Editor Wolfgang Walz University of Saskatchewan Saskatoon, SK, Canada
For further volumes: http://www.springer.com/series/7657
Cell Culture Techniques Second Edition
Edited by
Michael Aschner Department of Molecular Pharmacology, Albert Einstein College of Medicine, Bronx, NY, USA
Lucio Costa Department of Environment/STE 100, University of Washington, Seattle, WA, USA
Editors Michael Aschner Department of Molecular Pharmacology Albert Einstein College of Medicine Bronx, NY, USA
Lucio Costa Department of Environment/STE 100 University of Washington Seattle, WA, USA
ISSN 0893-2336 ISSN 1940-6045 (electronic) Neuromethods ISBN 978-1-4939-9227-0 ISBN 978-1-4939-9228-7 (eBook) https://doi.org/10.1007/978-1-4939-9228-7 © Springer Science+Business Media, LLC, part of Springer Nature 2019 This work is subject to copyright. All rights are reserved by the Publisher, whether the whole or part of the material is concerned, specifically the rights of translation, reprinting, reuse of illustrations, recitation, broadcasting, reproduction on microfilms or in any other physical way, and transmission or information storage and retrieval, electronic adaptation, computer software, or by similar or dissimilar methodology now known or hereafter developed. The use of general descriptive names, registered names, trademarks, service marks, etc. in this publication does not imply, even in the absence of a specific statement, that such names are exempt from the relevant protective laws and regulations and therefore free for general use. The publisher, the authors, and the editors are safe to assume that the advice and information in this book are believed to be true and accurate at the date of publication. Neither the publisher nor the authors or the editors give a warranty, express or implied, with respect to the material contained herein or for any errors or omissions that may have been made. The publisher remains neutral with regard to jurisdictional claims in published maps and institutional affiliations. This Humana Press imprint is published by the registered company Springer Science+Business Media, LLC, part of Springer Nature. The registered company address is: 233 Spring Street, New York, NY 10013, U.S.A.
Preface to the Series Experimental life sciences have two basic foundations: concepts and tools. The Neuromethods series focuses on the tools and techniques unique to the investigation of the nervous system and excitable cells. It will not, however, shortchange the concept side of things as care has been taken to integrate these tools within the context of the concepts and questions under investigation. In this way, the series is unique in that it not only collects protocols but also includes theoretical background information and critiques which led to the methods and their development. Thus, it gives the reader a better understanding of the origin of the techniques and their potential future development. The Neu
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