Association between the CEBPA and c-MYC genes expression levels and acute myeloid leukemia pathogenesis and development
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ORIGINAL PAPER
Association between the CEBPA and c-MYC genes expression levels and acute myeloid leukemia pathogenesis and development Adrian Krygier1 · Dagmara Szmajda‑Krygier1 · Aleksandra Sałagacka‑Kubiak1 · Krzysztof Jamroziak2 · Marta Żebrowska‑Nawrocka1 · Ewa Balcerczak1 Received: 27 September 2020 / Accepted: 27 October 2020 © The Author(s) 2020
Abstract CEBPA and c-MYC genes belong to TF and play an essential role in hematologic malignancies development. Furthermore, these genes also co-regulate with RUNX1 and lead to bone marrow differentiation and may contribute to the leukemic transformation. Understanding the function and full characteristics of selected genes in the group of patients with AML can be helpful in assessing prognosis, and their usefulness as prognostic factors can be revealed. The aim of the study was to evaluate CEBPA and c-MYC mRNA expression level and to seek their association with demographical and clinical features of AML patients such as: age, gender, FAB classification, mortality or leukemia cell karyotype. Obtained results were also correlated with the expression level of the RUNX gene family. To assess of relative gene expression level the qPCR method was used. The expression levels of CEBPA and c-MYC gene varied among patients. Neither CEBPA nor c-MYC expression levels differed significantly between women and men (p=0.8325 and p=0.1698, respectively). No statistically significant correlation between age at the time of diagnosis and expression of CEBPA (p=0.4314) or c-MYC (p=0.9524) was stated. There were no significant associations between relative CEBPA (p=0.4247) or c-MYC (p=0.4655) expression level and FAB subtype and mortality among the enrolled patients (p=0.5858 and p=0.8437, respectively). However, it was observed that c-MYC and RUNX1 expression levels were significantly positively correlated (rS=0.328, p=0.0411). Overall, AML pathogenesis involves a complex interaction among CEBPA, c-MYC and RUNX family genes. Keywords CEBPA gene · c-MYC gene · Transcription factors · Gene expression level · qPCR · AML · Adult leukemia
* Adrian Krygier [email protected] Dagmara Szmajda‑Krygier [email protected] Aleksandra Sałagacka‑Kubiak [email protected] Krzysztof Jamroziak [email protected] Marta Żebrowska‑Nawrocka [email protected] Ewa Balcerczak [email protected] 1
Laboratory of Molecular Diagnostics and Pharmacogenomics, Department of Pharmaceutical Biochemistry and Molecular Diagnostics, Medical University of Lodz, Muszynskiego 1 Street, 90‑151 Lodz, Poland
Department of Hematology, Institute of Hematology and Transfusion Medicine, Chocimska 5 Street, 00‑791 Warsaw, Poland
2
Introduction Acute myeloid leukemia (AML) belongs to the group of heterogeneous neoplastic diseases of the white blood cell system. AML is characterized by clonal proliferation and growth of cancer-transformed blast cells that originate from the precursor myeloid cell in the bone marrow and in the peripheral blood [1
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