Attenuation of sodium arsenite-induced cardiotoxicity and neurotoxicity with the antioxidant, anti-inflammatory, and ant
- PDF / 909,335 Bytes
- 14 Pages / 595.276 x 790.866 pts Page_size
- 67 Downloads / 139 Views
RESEARCH ARTICLE
Attenuation of sodium arsenite-induced cardiotoxicity and neurotoxicity with the antioxidant, anti-inflammatory, and antiapoptotic effects of hesperidin Müslüm Kuzu 1
&
Fatih Mehmet Kandemir 2 & Serkan Yıldırım 3 & Cüneyt Çağlayan 4 & Sefa Küçükler 2
Received: 26 June 2020 / Accepted: 18 October 2020 # Springer-Verlag GmbH Germany, part of Springer Nature 2020
Abstract In the scope of the study, the protective effect of hesperidin (HES), a flavanone glycoside, was investigated against sodium arsenite (NaAsO2, SA) induced heart and brain toxicity. For this purpose, 35 Sprague-Dawley male rats were divided into 5 different groups, 7 in each group. Physiological saline was given to the first group. Dose of 200 mg/kg of HES to the second group, 10 mg/kg dose of SA to the 3rd group, 100 mg/kg HES and 10 mg/kg SA to the 4th group, 200 mg/kg HES, and 10 mg/kg SA to the 5th group were given orally for 15 days. At the end of the study, biochemical, histopathological, and immunohistochemical examinations were performed on the heart and brain tissues of the rats. According to the results, SA increased malondialdehyde (MDA) and 8-hydroxy2′-deoxyguanosine (8-OHdG) levels and decreased glutathione (reduced, GSH) level and superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GPx) activities in both tissues. Also, it increased cardiac lactate dehydrogenase (LDH) and creatine kinase isoenzyme-MB (CK-MB) activities and cardiac troponin-I level (cTn-I), cerebral acetylcholine esterase activity, nuclear factor kappa-B (NF-κB), tumor necrosis factor-alpha (TNF-α), interleukin-one beta (IL-1β), and cysteine aspartate-specific protease-3 (caspase-3) levels. In addition, as a result of histopathological examination, it was determined that SA damaged tissue architecture, and as a result of immunohistochemical examination, it increased cardiac Bcl-2-associated X protein (Bax) and cerebral glial fibrillary acidic protein (GFAP) expression. The results have also shown that HES co-treatment has an antioxidant, antiinflammatory, antiapoptotic effect on SA-induced toxicity and aids to protect tissue architecture by showing a regulatory effect on all values. Consequently, it was determined that HES co-treatment had a protective effect on SA-induced heart and brain toxicity in rats. Keywords Sodium arsenite . Hesperidin . Cardiotoxicity . Neurotoxicity . Antioxidant . Inflammation . Apoptosis
Introduction Natural events as well as man-made processes cause the environment to be polluted by toxic components. Heavy metals Responsible Editor: Mohamed M. Abdel-Daim * Müslüm Kuzu [email protected] 1
Department of Nutrition and Dietetics, Faculty of Health Sciences, Karabuk University, Karabuk, Turkey
2
Department of Basic Sciences, Faculty of Veterinary Medicine, Ataturk University, Erzurum, Turkey
3
Department of Preclinical Sciences, Faculty of Veterinary Medicine, Ataturk University, Erzurum, Turkey
4
Department of Basic Sciences, Faculty of Veterinary Medicine, Bingöl University, Bingol, Turke
Data Loading...
