Augmenting the expression of accD and rbcL genes using optimized iron concentration to achieve higher biomass and biodie
- PDF / 976,321 Bytes
- 11 Pages / 547.087 x 737.008 pts Page_size
- 53 Downloads / 144 Views
(0123456789().,-volV) ( 01234567 89().,-volV)
ORIGINAL RESEARCH PAPER
Augmenting the expression of accD and rbcL genes using optimized iron concentration to achieve higher biomass and biodiesel in Chlorella vulgaris Atefeh Khamoushi . Vida Tafakori . Mohammad Ali Zahed . Somayeh Eghrari Gayglou . S. Abdolhamid Angaji
Received: 17 August 2019 / Accepted: 16 July 2020 Ó Springer Nature B.V. 2020
Abstract Chlorella vulgaris is a form of microalgae commonly employed as a biological source of oil for biodiesel production. Major algal cultivation strategies are focused on stimulating growth rate and lipid content. In the present study, the algal growth media was supplemented with iron (III) chloride (FeCl3), as a stimulating factor for growth and lipid production, in three iron concentrations including 90, 200, and 500 lM. The turbidity of algal cells was measured on different days, to determine the growth rate. In optimum iron concentration, this measurement experienced a 2.1-fold increase. Next, the lipid content was extracted, and the amount of lipid produced in each treatment was calculated, which demonstrated a 4.57fold increase in lipid productivity. The expression of genes corresponding to the metabolic enzymes (i.e. acetyl-CoA carboxylase (accD) and ribulose bisphosphate carboxylase large chain (rbcL)) was evaluated using real-time PCR under different initial iron feeds. As demonstrated in the results, the initial iron feed of 90 lM was an optimum concentration that obtained the highest growth rate, more cell density, and increased lipid production. In 90 lM initial iron concentration, the expression of accD and rbcL genes showed a 4.8- and 35-fold increase, respectively,
A. Khamoushi V. Tafakori (&) M. A. Zahed S. E. Gayglou S. A. Angaji Department of Cell and Molecular Biology, Faculty of Biological Sciences, Kharazmi University, Tehran, Iran e-mail: [email protected]; [email protected]
compared to that of the control genes. Based on the results, this optimum iron concentration could satisfy the industrial interest in biodiesel production from C. vulgaris as a potential stimulating factor. However, higher levels of iron (e.g. 200 and 500 lM) failed to act as positive stress for increasing biodiesel production. Finally, in this paper, different mechanisms where iron affects acetyl-CoA carboxylase (ACCase) and 1,5-ribulose bisphosphate carboxylase/oxygenase (RuBisCo) are illustrated. Keywords RuBisCo
Biofuel Lipid Microalgae ACCase
Introduction Nowadays, the concerns raised by fossil fuels as key contributors to air pollution, and their shortage, necessitates considerations to replace these sources of energies with alternative sources. Biodiesel is commonly suggested as an alternative to fossil fuels and is produced from the plant- or animals-based materials (Griffiths and Harrison 2009). Microalgae are the best source of biodiesel production due to their numerous advantages over terrestrial plants (Gong and Jiang 2011). One advantage of microalgae is the high oil content in their cell
Data Loading...
