Autologous mesenchymal stem cell endografting in experimental cerebrovascular aneurysms
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INTERVENTIONAL NEURORADIOLOGY
Autologous mesenchymal stem cell endografting in experimental cerebrovascular aneurysms Aymeric Rouchaud & Clément Journé & Liliane Louedec & Véronique Ollivier & Mohammed Derkaoui & Jean-Baptiste Michel & Mikael Mazighi
Received: 26 October 2012 / Accepted: 4 March 2013 / Published online: 21 March 2013 # Springer-Verlag Berlin Heidelberg 2013
Abstract Introduction Coiling is the gold standard for the treatment of intracranial aneurysms. However, some issues associated with endovascular treatment limit its long-term efficiency. Recanalization with coil compaction is certainly the most important. New approaches may be considered to promote thrombus colonization by mesenchymal cells and aneurysm healing. In the present study, we have percutaneously delivered autologous bone marrow mesenchymal stem cells (BMSCs) to an elastase-induced rabbit carotid aneurysm model in vivo. Methods Autologous mesenchymatous stem cells were obtained after femoral puncture and bone marrow aspiration. After 2 weeks of in vitro cell culture, five million BMSCs were grafted in the carotid aneurysm using an endovascular approach. Results We demonstrated the feasibility of in vivo percutaneous seeding of autologous BMSCs in the aneurysm by positive Hoechst fluorostaining. Two weeks later, conventional angiography showed an increase in median aneurysmal surface in the sham group, whereas this surface was decreased in A. Rouchaud : C. Journé : L. Louedec : V. Ollivier : M. Derkaoui : J.-B. Michel : M. Mazighi INSERM Research Unit 698, Inserm‐Paris 7 Denis Diderot University, Paris, France A. Rouchaud : M. Mazighi Department of Neurology and Stroke Centre, Paris 7‐Denis Diderot Sorbonne University, 46 rue Henri Huchard, 75018 Paris, France A. Rouchaud (*) INSERM U‐698, Department of Neurology and Stroke Centre, Bichat University Hospital, 46 rue Henri Huchard, 75018 Paris, France e-mail: [email protected]
the group treated with BMSCs, +28.4 versus −26.4 %, respectively (p=0.01). BMSC seeding resulted in intimal hyperplasia with cell colonization and disappearance of the thrombus. Conclusion In conclusion, percutaneous seeding of BMSCs may colonize and heal the arterial wall thus limiting aneurysm expansion. Keywords Rabbit . Carotid aneurysm . Mesenchymal stem cells . Cell therapy . Elastase-induced aneurysms Abbreviations BMSC Bone marrow mesenchymal stem cells CFA Common femoral artery PCA Procoagulant activity RCCA Right common carotid artery TF Tissue factor
Introduction Endovascular therapy of intracranial aneurysms is now the gold standard [26] and has supplanted the surgical approach in numerous centers [26]. However, recurrences after coiling of intracranial aneurysms remain the main issue, and are associated with a potential risk of late rebleeding [7, 27, 34, 35]. The recanalization phenomenon attributable to coil compaction is particularly observed in wide-necked or large aneurysms [17, 33]. Several “mechanical” approaches have been attempted to reduce recanalization of aneurysms such as incr
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