Basic Fibroblast Growth Factor Attenuates Bisphosphonate-Induced Oxidative Injury but Decreases Zinc and Copper Levels i
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Basic Fibroblast Growth Factor Attenuates BisphosphonateInduced Oxidative Injury but Decreases Zinc and Copper Levels in Oral Epithelium of Rat Gülperi Koçer & Mustafa Nazıroğlu & Ömer Çelik & Levent Önal & Derviş Özçelik & Murat Koçer & Tolga Taha Sönmez
Received: 28 February 2013 / Accepted: 27 March 2013 / Published online: 10 April 2013 # Springer Science+Business Media New York 2013
Abstract Recent studies have reported oxidative damage due to bisphosphonate (BP) in various cancer tissues and neurons, although basic fibroblast growth factor (bFGF) induced antioxidant effects in the cells. The bFGF may modulate the BPinduced oxidative stress in oral epithelium of rats. This study was undertaken to explore possible beneficial antioxidant effects of bFGF on oxidative stress induced by BP in oral epithelium of rats. Twenty-eight rats were equally divided into four groups. The first group was used as control. The second, third and fourth groups intraperitoneally received BP (zoledronic acid), bFGF and BP + bFGF. At the end of 10 weeks, the rats were sacrificed, and oral epithelium samples were taken for analyses. In BP group, the lipid peroxidation G. Koçer : L. Önal Department of Oral and Maxillofacial Surgery, Dentistry Faculty, Suleyman Demirel University, Isparta, Turkey M. Nazıroğlu (*) : Ö. Çelik Neuroscience Research Center, Suleyman Demirel University, Tıp Fakültesi Binasi, TR-32260 Isparta, Turkey e-mail: [email protected] M. Nazıroğlu : Ö. Çelik Department of Biophysics, Medical Faculty, Suleyman Demirel University, Isparta, Turkey D. Özçelik Department of Biophysics, Medical Faculty, Cerrahpasa Medical Faculty, Istanbul University, Istanbul, Turkey
levels were increased in the oral epithelium, while the activities of glutathione peroxidase (GSH-Px) and the concentrations of total antioxidant status (TAS) were decreased. In rats treated with bFGF, lipid peroxidation levels decreased, and the activities of GSH-Px and concentrations of TAS improved in the oral epithelium. However, zinc and copper levels were decreased in the oral epithelium by BP and bFGF treatments. Concentrations of vitamin E and reduced glutathione in the samples did not change in the groups. In conclusion, treatment with bFGF modulated the antioxidant redox system and reduced the oral epithelium oxidative stress induced by BP. However, zinc and copper levels were decreased by BP and bFGF treatments. Keywords Biphosphanate . Oxidative defense . Copper . Zinc . Oral epithelium . Basic fibroblast growth factor
List of Abbreviations bFGF Basic fibroblast growth factor BP Bisphosphonate Cu Copper GSH Glutathione GSH-Px Glutathione peroxidase MDA Malondialdehyde ROS Reactive oxygen species SOD Superoxide dismutase TAS Total antioxidant status Zn Zinc
M. Koçer Department of Medical Oncology, Medical Faculty, Suleyman Demirel University, Isparta, Turkey
Introduction
T. T. Sönmez Department of Oral and Maxillofacial Surgery, Medical Faculty, RWTH Aachen University, Aachen, Germany
Bisphosphonate (BP) is chemical analogue of inorg
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