Bioactivity-Oriented Purification of Polyphenols from Cinnamomum cassia Presl. with Anti-Proliferation Effects on Colore
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ORIGINAL PAPER
Bioactivity-Oriented Purification of Polyphenols from Cinnamomum cassia Presl. with Anti-Proliferation Effects on Colorectal Cancer Cells Li Gao 1 & Na Gou 1 & Erdong Yuan 1 & Jiaoyan Ren 1 Accepted: 13 August 2020 # Springer Science+Business Media, LLC, part of Springer Nature 2020
Abstract Cinnamomum cassia Presl. (CCP) is a popular natural spice possessing various pharmacological properties. We obtained polyphenol-rich fraction (CCP-P) from CCP by bioactivity-oriented purification method and evaluated its Wnt signaling inhibition activity. Firstly, the phenolic components were identified as the main bioactive compounds with anti-colorectal cancer activity. Then, we compared the anti-colorectal cancer activity of CCP extract obtained from different solvent by cell morphology alteration and EdU assay. Ethanol extract showed higher antiproliferative activity compared to water extract on HCT116 cells, with proliferating cells reducing to 41.12 and 21.83% at 156.00 μg GAE/mL, respectively. Next, separation and enrichment of polyphenols from ethanol extract was performed on AB-8 macroporous resins under optimal conditions. Further evaluation of the CCP-P bioactivity revealed that it exerted more potent antiproliferative activity on RKO and HCT116 cells, showing higher selectivity for Wnt-dependent colorectal cancer cells (CRCs). Ten major polyphenols were identified in the CCP-P by UPLCESI-MS/MS. In summary, this study presents evidence that CCP-derived polyphenols are promising potential candidates as functional food ingredients against CRC. Keywords Cinnamomum cassia Presl. . Polyphenols . Anti-proliferation . Colorectal cancer cells . Wnt signaling
Abbreviations BV Bed volume CCP Cinnamomum cassia Presl CCP-P Cinnamomum cassia Presl. polyphenols rich faction CRC Colorectal cancer CRCs Colorectal cancer cells GAE Gallic acid equivalents MTT 3-(4,5-dimethylthiazol-2-yl) -2,5-diphenyltetrazolium bromide TPC Total phenolic content Wnt Wingless / Integrated
Electronic supplementary material The online version of this article (https://doi.org/10.1007/s11130-020-00846-8) contains supplementary material, which is available to authorized users. * Jiaoyan Ren [email protected] 1
School of Food Science and Engineering, South China University of Technology, Guangzhou 510640, Guangdong, China
Introduction Colorectal cancer (CRC) is a major public health issue, becoming the second most common cancer in women and the third most common cancer in men worldwide [1] More than 90% of all CRC arise from hyperactive canonical Wnt signaling [2]. The abnormally activated Wnt signaling in CRC makes the pathway an effective target for the management of CRC [3]. Some chemical synthetic compounds targeting Wnt signaling have been applied in the treatment of CRC [3]. Considering the disadvantages of these drugs including the adverse side effects, toxicity and high cost, there is an urgent need to develop safe and effective dietary agents from natural products to help manage CRC. In this regard, dietary polyphenols are cont
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