Biocatalytic Activity of Polyethylene Imine Hydrogel Complexes with Transition Metal Ions
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0897-J03-11.1
Biocatalytic Activity of Polyethylene Imine Hydrogel Complexes with Transitional Metal Ions Gulnara A. Bektenova, Nurzhan S. Chinibayeva and Essen A. Bekturov Institute of Chemical Sciences, 050010, Almaty, Walikhanov str., 106, Republic of Kazakhstan, ABSTRACT Polyethylene imine hydrogel complexes with various salts of ferric and ferrous iron and also with complex salts of iron as well as insoluble ternary complexes of various composition: low cross-linked polyethylene imine hydrogel - copper (II) nitrate - sodium dodecylbenzylsulfate have been received. Their biocatalytic properties in the reaction of hydrogen peroxide decomposition (catalase activity) have been investigated. It was shown that catalase activity of complexes depends on the salts type and valence state of iron ions. The complexes of ferric iron as well as ternary complexes are most active in the reaction of hydrogen peroxide decomposition.
INTRODUCTION Nowadays researchers have paid much of their increasing attention to biomimetic problems recently. The studying object of biomimetic is considered to be the system which is imitating all the nature process, including various functions, such as biocatalytic ones. Special attention is given to the enzyme-like systems creation which are working according to the metal-containing enzymes and having almost the same characteristics on their activity and selectivity. Therefore up till now the researchers have been interested in studying processes of the model systems which are simulating catalase enzyme activity and catalyzing the reaction of hydrogen peroxide decomposition; there are three important reasons which are increasing their attention: firstly, catalase - one of the most effective enzymes with great number of revolutions; secondly, through the model creation process it is possible to reach its activity approximately to catalase activity; thirdly, on catalase example it is possible to track the iron ion ligand environment influence as well as to define a role of apoenzyme in the catalyst activity[1]. High-swelling polymer hydrogels are rather perspective in catalysis. On the basis of such hydrogels it is possible to get transitional metal complexes with various structure and composition; more over the hydrogel ability to swelling process causes its high permeability for reagents, and a polymer net mobility can provide the realization process of the catalyst direction on a substrate. The nature of linear macromolecules interaction and their low-cross-linked analogues with the ions of metals can be ionic, coordination or ionic and coordination [2], that is defined by the state of macromolecules functional groups. EXPERIMENTAL DETAILS As the test object we have chosen polyethylene imine hydrogel (PEIH) and different iron salts that is connected with the catalase structure that has so-called “natural mark” in prosthetic group – heme iron.
0897-J03-11.2
Polyethylene imine hydrogel complexes was synthesized in dimethylformamide adding epichlorhydrin to the linear polymer up to the gel fo
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