Biocompatibility, mechanical, and bonding properties of a dental adhesive modified with antibacterial monomer and cross-
- PDF / 1,575,020 Bytes
- 13 Pages / 595.276 x 790.866 pts Page_size
- 28 Downloads / 240 Views
ORIGINAL ARTICLE
Biocompatibility, mechanical, and bonding properties of a dental adhesive modified with antibacterial monomer and cross-linker Hoda Moussa 1 & Megan M. Jones 2 & Ningbo Huo 3 & Runsheng Zhang 3 & Mayuresh Keskar 3 & Michelle B. Visser 2 & Mark T. Swihart 3 & Chong Cheng 3 & Camila Sabatini 4 Received: 22 May 2020 / Accepted: 23 September 2020 # Springer-Verlag GmbH Germany, part of Springer Nature 2020
Abstract Objectives This study investigated the antibacterial, cytotoxicity, and mechanical properties of a dental adhesive modified with quaternary ammonium monomer ((2-acryloyloxyethyl)dimethyldodecylammonium bromide) and cross-linker (bis(2acryloyloxyethyl)methyldodecylammonium bromide). Materials and methods Monomer (M), cross-linker (C), or a combination of these (M + C) were incorporated into adhesive Adper Single Bond Plus (SB) in 5, 10, or 25% (as wt%). A colony-forming unit and MTT assays were used to evaluate antibacterial properties against Streptococcus mutans and cell viability. Resin-dentin beams (0.9 ± 0.1 mm2) were evaluated for micro-tensile bond strength (μTBS) after 24 h, 6 months, and 3 years. Hourglass specimens were evaluated for ultimate tensile strength (UTS) after 24 h, 1 week, and 6 months. Micro-hardness measurements after softening in ethanol were taken as an indirect assessment of the polymer crosslinking density. Kruskal-Wallis, one-way ANOVA, two-way ANOVA, and Student’s t test were used for analysis of the antibacterial, cytotoxicity, μTBS, UTS, and hardness data, all with a significance level of p < 0.05. Results 10%M and 25%M demonstrated a significant reduction in S. mutans relative to SB (p < 0.001). No differences in cytotoxicity were detected for any of the groups. After 6 months, no changes in μTBS were shown for any of the groups. After 3 years, all groups evidenced a significant decrease in μTBS (p < 0.05) except 5%M, 5%C, and 5%M + 5%C. All groups demonstrated either stable or significantly increased UTS after 6 months. Except for the cross-linker groups, a significant decrease in micro-hardness was shown for all groups after softening in ethanol (p < 0.05). Conclusions A 5–10% of monomer may render the resin antibacterial without a compromise to its mechanical and bonding properties. Clinical relevance Biomodification of a resin adhesive with an antibacterial monomer and cross-linker may help improve the life span of adhesive restorations. Keywords Adhesion . Antibacterial . Bond strength . Cross-linker . Monomer
* Camila Sabatini [email protected]
Mark T. Swihart [email protected]
Hoda Moussa [email protected]
Chong Cheng [email protected]
Megan M. Jones [email protected]
1
Ningbo Huo [email protected]
Department of Chemistry, Buffalo State College, 1300 Elmwood Avenue, Buffalo, NY 14222, USA
2
Department of Oral Biology, School of Dental Medicine, University at Buffalo, 3435 Main Street, Buffalo, NY 14214, USA
Runsheng Zhang [email protected]
3
Department of Chemical and Biological Engineering, School of Engineering, Universit
Data Loading...
