Cell lines authentication and mycoplasma detection as minimun quality control of cell lines in biobanking
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Cell lines authentication and mycoplasma detection as minimun quality control of cell lines in biobanking C. Corral-Va´zquez . R. Aguilar-Quesada . P. Catalina . ´ vila G. Lucena-Aguilar . G. Ligero . B. Miranda . J. A. Carrillo-A
Received: 20 July 2016 / Accepted: 23 February 2017 Ó The Author(s) 2017. This article is published with open access at Springerlink.com
Abstract Establishment of continuous cell lines from human normal and tumor tissues is an extended and useful methodology for molecular characterization of cancer pathophysiology and drug development in research laboratories. The exchange of these cell lines between different labs is a common practice that can compromise assays reliability due to contamination with microorganism such as mycoplasma or cells from different flasks that compromise experiment reproducibility and reliability. Great proportions of cell lines are contaminated with mycoplasma and/or are replaced by cells derived for a different origin during processing or distribution process. The scientific community has underestimated this problem and thousand of research experiment has been done with cell lines that are incorrectly identified and wrong scientific conclusions have been published. Regular contamination and authentication tests are necessary in order to avoid negative consequences of widespread misidentified and contaminated cell lines. Cell banks generate, store and distribute cell lines for research, being mandatory a consistent and continuous quality program. Methods implementation for guaranteeing
C. Corral-Va´zquez R. Aguilar-Quesada P. Catalina G. Lucena-Aguilar G. Ligero ´ vila (&) B. Miranda J. A. Carrillo-A Andalusian Public Health System Biobank, Avenida Del Conocimiento S/N, 18016 Granada, Spain e-mail: [email protected]; [email protected]
both, the absence of mycoplasma and authentication in the supplied cell lines, has been performed in the Andalusian Health System Biobank. Specifically, precise results were obtained using real time PCR detection for mycoplasma and 10 STRs identification by capillary electrophoresis for cell line authentication. Advantages and disadvantages of these protocols are discussed. Keywords STRs Cell line authentication Mycoplasma Quality control Biobanking PCR
Introduction The use of cultured cells that acquired the ability to proliferate indefinitely is an extended tool in research laboratories. Cell lines are used as in vitro models of health and disease by retaining many of the properties of the parental tissue or cell type, including diseasespecific changes (Christine Alston-Roberts et al. 2010; Shannon et al. 2016). Because of those reasons, cell lines based screening platforms are excellent models to test new therapeutic approaches. Nowadays is frequent the exchange of established cells between different laboratories as result of groups interactions. That practice involves a high risk of cell lines contamination by two common sources: a microorganism, usually mycoplasma, or a foreign cel
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