Cell therapy as a new approach on hepatic fibrosis of murine model of Schistosoma mansoni -infection
- PDF / 3,287,905 Bytes
- 10 Pages / 595.276 x 790.866 pts Page_size
- 21 Downloads / 192 Views
ORIGINAL PAPER
Cell therapy as a new approach on hepatic fibrosis of murine model of Schistosoma mansoni‑infection Muslimah Alsulami1 · Rewaida Abdel‑Gaber2,3 Received: 27 June 2020 / Accepted: 11 August 2020 © Witold Stefański Institute of Parasitology, Polish Academy of Sciences 2020
Abstract Background Schistosomiasis is an acute and chronic disease of the genus Schistosoma triggered by blood flukes. Schistosomiasis is a disease occurring in, or endemic to, tropical and subtropical regions. A new concept was implemented to deal with schistosomiasis from natural plant sources. Curcumin’s common name is Turmeric. Curcumin has proven to be main active component in Curcuma longa L. and has a wide range of anti-phrastic effects. Previous studies have shown the role of bone marrow mesenchymal stem cells (BMSCs) therapy in hepatic fibrosis recovery. Objective The current study was, therefore, intended to examine therapeutic role of BMSCs and Turmeric in murine schistosomiasis mansoni. Animals Mice were divided into five groups: a negative control group (non-infected non-treated), a positive control group (infected non-treated), a BMSCs treated group; Turmeric treated group, and untreated group. BMSCs derived from male mice were injected intraperitoneally into female mice receiving S. mansoni cercariae through the subcutaneous route. Liver histopathology and immuno-histochemical examinations were evaluated. Results BMSCs intraperitoneal injection resulted in a significant reduction of liver collagen, granuloma size, and significant increase of OV-6 expression in the Schistosomiasis-treated mice group. There was overall improvement in pathological changes of the liver. Unfortunately, group IV showed a mild improvement in the granuloma size and fibrosis compared to corresponding BMSCs treatment group, although with vacuolated liver cells. Conclusion and Clinical Relevance BMSCs have a regenerative potential in liver tissue histopathology by decreasing liver fibrosis and granulomas. Turmeric, by contrast, could not be used as an anti-fibrotic, according to the findings. Keywords BMSCs · Histopathology · Schistosoma mansoni · Turmeric · OV-6
Introduction A trematode of Schistosoma causes schistosomiasis, which is considered a chronic debilitating disease. Schistosomiasis is considered the second disease after malaria infection in terms of its relevance to public health, economic, and social effects. According to reports, at least 207 million people are globally infected while 779 million people are still at risk * Rewaida Abdel‑Gaber [email protected] 1
Biology Department, College of Science, University of Jeddah, Jeddah, Saudi Arabia
2
Zoology Department, College of Science, King Saud University, Riyadh, Saudi Arabia
3
Zoology Department, Faculty of Science, Cairo University, Cairo, Egypt
[41]. World Health Organization (WHO) has indicated more than 200,000 schistosomiasis deaths are reported annually in sub-Saharan Africa [44]. Schistosoma mansoni infection is the most frequent source of schis
Data Loading...
