Central role of 70-kDa heat shock protein in adaptation of plants to drought stress
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ORIGINAL PAPER
Central role of 70-kDa heat shock protein in adaptation of plants to drought stress Peyman Aghaie 1 & Seyed Ali Hosseini Tafreshi 2 Received: 28 April 2020 / Revised: 16 July 2020 / Accepted: 22 July 2020 # Cell Stress Society International 2020
Abstract The 70-kDa heat shock proteins (HSP70s) are a conserved class of chaperones that play critical roles during the normal life cycle of plants. HSP70s are particularly involved in the regulation of biotic and abiotic stress responses. In this paper, the potential roles of this protein were investigated. A reverse genetic approach was employed for transient silencing of hsp70 gene in tomato (Solanum lycopersicum L.) to evaluate different growth and physiological parameters under normal conditions and during the response to drought stress. A combined ANOVA (analysis of variance) and HCA (hierarchical clustering analysis) showed that hsp70 silencing led to severe growth retardation and mortality, significant membrane damage and leakage, decline in relative water content, low rate of pigment accumulation, and reduced antioxidant enzyme activity under normal and drought stress conditions. Among the different parameters, proline was the only trait that was unaffected by gene silencing and accumulated by similar amounts to that of nonsilent plants. In conclusion, HSP70 played critical roles in maintaining the cellular homeostasis of plants during adaptation to drought and under normal plant life conditions. It was speculated that proline was, to some extent, involved in improving the loss of protein folding or function resulting from HSP70 deficiency, and played a crucial role in the adaptation of plants on exposure to stress. Keywords Heat shock proteins (HSP) . Hsp70s . Virus induced gene silencing (VIGS) . Tomato . Drought stress
Abbreviations ANOVA Analysis of variance AsA Ascorbic acid APX Ascorbate peroxidase Car Carotenoid CAT Catalase Chl Chlorophyll CRD Completely randomized design DW Dry weight EC Electrical conductivity EL Electrolyte leakage FW Fresh weight GFP Green fluorescent protein
* Seyed Ali Hosseini Tafreshi [email protected] 1
Department of Biology, Faculty of Science, Payame Noor University, PO Box 19395-3697, Tehran, Iran
2
Biotechnology Division, Department of Cell and Molecular Biology, Faculty of Chemistry, University of Kashan, Kashan 8731753153, Iran
HCA HSP MDA NaOCl NBT ROS RWC SOD TBA TCA TRV TW
Hierarchical clustering analysis Heat shock proteins Malondialdehyde Sodium hypochlorite Nitro blue tetrazolium Reactive oxygen species Relative water content Superoxide dismutase Thiobarbituric acid Trichloroacetic acid Tobacco rattle virus Turgid weight
Introduction The tomato plant (Solanum lycopersicum L.) is an important economic crop, and is widely cultivated throughout the world. The species has been considered as a model in studies to transfer agronomically important traits by introducing new genes (Paduchuri et al. 2010). One of the desired traits for crop plants is their adaptation to stress conditions, such as
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