Characterization and identification of OsFTL8 gene in rice
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Plant Biotechnology Reports https://doi.org/10.1007/s11816-020-00644-3
ORIGINAL ARTICLE
Characterization and identification of OsFTL8 gene in rice Siju Zhang1 · Yajun Jin1 · Hongjiao Hao1 · Shanshan Liang1 · Xuan Ma1 · Weijiang Luan1 Received: 28 June 2020 / Accepted: 4 October 2020 © Korean Society for Plant Biotechnology 2020
Abstract The rice genome contains 13 Flowering Locus T-Like (FT-Like) genes, among which, OsFTL2/Hd3a and OsFTL3/RFT1 are characterized as florigen-encoding genes, promoting rice flowering under short-day and long-day conditions, respectively. However, the functions of the other FT-Like members are still unknown in rice. In this study, we characterized and identified a member of FT-Like family, OsFTL8 gene. The investigation of photoperiodic expression pattern showed that OsFTL8 transcription was mainly induced under short-day conditions. The qRT-PCR and GUS staining assays indicated that OsFTL8 was broadly expressed in various organs, including stems, leaves and panicles. OsFTL8 overexpression transgenic lines and its knockout mutants displayed no obvious change in their heading date, compared with the wild type. However, the pollen viability and seed setting rate were decreased in the overexpression transgenic lines. OsFTL8 localizes in both nucleus and cytoplasm. And we found that OsFTL8 does not interact with OsFD1 in vivo, suggesting that OsFTL8 may not be the component of florigen activation complex (FAC). In summary, our results indicate that OsFTL8 is associated with pollen viability and not involved in regulating rice flowering time, implying functional divergence of FT-Like members. Keywords Rice · Flowering time · Pollen viability · PEBP · OsFTL8
Introduction Flowering, a remarkable transition from vegetative growth to reproductive growth of higher plants, is mainly induced by environmental signal such as photoperiod and regulated by the expression of endogenous florigen genes (Song and Luan 2012). Florigen is a kind of small globular protein, belonging to the phosphatidylethanolamine-binding protein (PEBP) family (Chardon and Damerval 2005). The PEBP genes are conserved among various plant species, from monocots to dicots (Karlgren et al. 2011; Lorenzo et al. 2020; Sun et al. 2018). Rice is a facultative short-day plant, and its flowering is promoted and repressed by short-day and long-day, respectively (Cai et al. 2019). There are 19 PEBP genes Electronic supplementary material The online version of this article (https://doi.org/10.1007/s11816-020-00644-3) contains supplementary material, which is available to authorized users. * Weijiang Luan [email protected] 1
College of Life Sciences, Tianjin Key Laboratory of Animal and Plant Resistance, Tianjin Normal University, Tianjin 300387, People’s Republic of China
in rice genome, which are further divided into FT-like (FTL), TF-like (TFL) and Mother of FT (MFT) subfamilies, based on the deduced protein similarity, which include 13 (OsFTL1 ~ OsFTL13), 4 (RCN1 ~ RCN4) and 2 (OsMFT1 and OsMFT2) members, respectively (Kabaya
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