Characterization and validation of long noncoding RNAs as new candidates in prostate cancer
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PRIMARY RESEARCH
Cancer Cell International Open Access
Characterization and validation of long noncoding RNAs as new candidates in prostate cancer Shengyang Ge1†, Yuanyuan Mi2†, Xiaojun Zhao3, Qingfeng Hu1, Yijun Guo4, Fan Zhong5, Yang Zhang5, Guowei Xia1* and Chuanyu Sun1*
Abstract Background: Long noncoding RNAs (lncRNAs) have been proved to be an important regulator in gene expression. In almost all kinds of cancers, lncRNAs participated in the process of pathogenesis, invasion, and metastasis. Meanwhile, compared with the large amounts of patients, there is rare knowledge about the role of lncRNAs in prostate cancer (PCa). Material/Method: In this study, lncRNA expression profiles of prostate cancer were detected by Agilent microarray chip, 5 pairs of case and control specimens were involved in. Differentially expressed lncRNAs were screened out by volcano plot for constructing lncRNA-miRNA-mRNA central network. Then, the top ten up-regulated and downregulated lncRNAs were validated by qRT-PCR in another 5 tumor specimens and 7 para-cancerous/benign contrasts. Furthermore, we searched for the survival curve of the top 10 upregulated and downregulated lncRNAs. Results: A total of 817 differentially expressed lncRNAs were filtered out by the criteria of fold change (FC) and t-test p
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