Characterization of microsatellite loci developed for the Mexican four-eyed octopus Octopus maya
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TECHNICAL NOTE
Characterization of microsatellite loci developed for the Mexican four-eyed octopus Octopus maya Oscar E. Jua´rez • Carlos Rosas • Leticia Arena Luis Enrı´quez • Faustino Camarena • Niall McKeown • Paul W. Shaw
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Received: 8 March 2013 / Accepted: 15 March 2013 / Published online: 23 March 2013 Ó Springer Science+Business Media Dordrecht 2013
Abstract Ten polymorphic microsatellite DNA loci were isolated from Mexican four-eyed octopus Octopus maya, by construction of an enriched genomic library. Genotyping of 35 individuals from Sabancuy Campeche, Me´xico, revealed variable levels of locus polymorphism with an average of 9.2 alleles. The observed and expected heterozygosity per locus ranged from 0.53 to 0.93 and from 0.48 to 0.87, respectively. No evidence of linkage disequilibrium was detected between pairs of loci and genotype proportions at all loci conformed to Hardy–Weinberg equilibrium expectations. The microsatellite loci developed constitute a suite of genetic markers applicable to sustainable fishery management for O. maya. Keywords Octopus maya Polymorphic microsatellite Fishery management
O. E. Jua´rez (&) Departamento de Biotecnologı´a Marina, Centro de Investigacio´n Cientı´fica y de Educacio´n Superior de Ensenada, Carretera Ensenada-Tijuana # 3918, Ensenada, Baja California, Me´xico e-mail: [email protected] C. Rosas L. Arena Unidad Multidisciplinaria de Docencia e Investigacio´n, Facultad de Ciencias, Universidad Nacional Auto´noma de Me´xico, Puerto de abrigo s/n, Sisal, Yucata´n, Me´xico L. Enrı´quez F. Camarena Universidad Auto´noma de Baja California, Km. 103 Carretera Tijuana-Ensenada, Ensenada, Baja California, Me´xico N. McKeown P. W. Shaw Institute of Biological, Environmental and Rural Sciences, Aberystwyth University, Penglais, Aberystwyth, Ceredigion SY23 3BF, United Kingdom
The endemic Octopus maya represents one of the most important fisheries in Yucatan peninsula, Mexico. The fishery has been managed as a single unit, but emerging evidence suggests that perhaps there are different units and that the resource could be overexploited (Jua´rez et al. 2010). The high intrapopulation variability of microsatellite markers and their relatively ease of genotyping, makes them a powerful tool to discriminate cephalopod populations, and informing sustainable fishery management (Shaw et al. 1999). The isolation and characterization of the first microsatellite loci for O. maya are reported here. Microsatellites were isolated an enriched partial genomic library by method outlined by Glenn and Schable (2005). The genomic DNA was extracted from O. maya muscle tissue following phenol:chloroform (1:1) method, subsequently DNA was digested with restriction Rsa-I enzyme (New England Biolabs) producing fragments suitable for the ligation of oligonucleotide linkers specific to the protocol. Linker-ligated DNA was amplified by polymerase chain reaction (PCR). Enrichment was then performed by selective hybridisation of biotin-labelled repeat motif oligonucleotide probes to the
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