Characterization of Platelet-Rich Plasma/Gellan Gum Hydrogel Composite for Biological Performance to Induce Chondrogenes
- PDF / 2,628,030 Bytes
- 6 Pages / 595 x 842 pts (A4) Page_size
- 48 Downloads / 193 Views
Article
DOI 10.1007/s13233-020-8155-4
www.springer.com/13233 pISSN 1598-5032 eISSN 2092-7673
Characterization of Platelet-Rich Plasma/Gellan Gum Hydrogel Composite for Biological Performance to Induce Chondrogenesis from Adipose-Derived Stem Cells Namyeong Kim† Joo Hee Choi† Min Joung Choi Jin Su Kim Wooyoup Kim Jeong Eun Song Gilson Khang*
Department of PolymerNano Science & Technology and Polymer Materials Fusion Research Center, Department of Bionanotechnology and Bio-Convergence Engineering, Jeonbuk National University, 567 Baekje-daero, Deokjin-gu, Jeonju-si, Jeonbuk 54896, Korea
Received April 5, 2020 / Revised August 11, 2020 / Accepted August 29, 2020
Abstract: In this experiment, hydrogel composite was developed with gellan gum (GG) and platelet-rich plasma (PRP) to induce chondrogenesis of adipose-derived stem cells (ADSCs) in the hydrated environment. FTIR, SEM, and compression test were carried out to characterize the fabricated material. Biocompatibility was analyzed by MTT assay. The morphology of the encapsulated ADSCs was observed under SEM. Chondrogenesis was examined by PCR. The chemical and morphological analysis displayed the appearance of PRP in the matrix. The mechanical property of the hydrogel decreased as the PRP was loaded but viability and chondrogenesis were significantly increased with the proper amount of the PRP. Overall, the composite of the appropriate incorporation of PRP in the GG can enhance the microenvironment for chondrogenesis and expected to have great potential in tissue engineering. Keywords: adipose-derived stem cells, gellan gum, platelet-rich plasma, chondrogenesis.
1. Introduction Mesenchymal stem cells (MSCs) possess high potential because of their multipotential, self-renewal capacity, and can avoid cancer metastasis and ethical dilemma. Methods to induce differentiation from stem cells include co-culture or inclusion of growth factors and biomolecules. However, these methods are expensive and may be temporal. Another strategy to generate differentiation of MSCs can be providing a microenvironment for stem cell niche.1 Hydrogels mimic the microenvironment of the extracellular matrix in the chemical and physical aspect. Therefore, they are an ideal cellular environment for stem cell differentiation. Polysaccharides hydrogels are biocompatible and biodegradable polymers that are widely applied in tissue engineering.2,3 Among various types of polysaccharides hydrogels, gellan gum hydrogel (GG) has been suggested as a promising biomaterial for cartilage tissue engineering (TE) applications.4-9 Its versatility and efficacy have been reported and demonstrated for cartilage Acknowledgments: This research was supported by the Korea Health Technology R&D Project through the Korea Health Industry Development Institute (KHIDI), funded by the Ministry of Health &Welfare, Republic of Korea (HI15C2996) and the International Research & Development Program of the National Research Foundation of Korea (NRF) funded by the Ministry of Science, ICT & Future Planning (NRF-2017K1A3
Data Loading...
