Chemiluminescent Reaction of N -Octyl Luminol with a Hypochlorite Ion in a Micellar Medium
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iluminescent Reaction of N-Octyl Luminol with a Hypochlorite Ion in a Micellar Medium T. V. Yankovaa, *, P. V. Melnikovb, and N. K. Zaytsevb a
Research and Technical Center of Radiation and Chemical Safety and Hygiene, Federal Medical-Biological Agency, Moscow, 123182 Russia bMIREA—Russian Technological University, Moscow, 119454 Russia *e-mail: [email protected] Received September 10, 2019; revised October 12, 2019; accepted November 14, 2019
Abstract—We study the effect of surfactants having a different charge and length of the hydrocarbon radical on the intensity and kinetic laws of the model chemiluminescent reaction of a hydrophobic analogue of luminol, N-octyl luminol. The effect of the solution pH and the charge, structure, and concentration of surfactants on the intensity of the chemiluminescence is assessed, and the kinetic laws of the chemiluminescent reaction are determined. Keywords: chemiluminescence, micellar systems, luminol, luminol analogue, hypochlorite ion, surfactants DOI: 10.3103/S0027131420050090
Chemiluminescent reactions are widely used in diagnostic systems; they are the base of various analytical methods [1–5]. Many real objects of analysis, especially biological ones, such as milk or blood components, often contain surface-active components [6]. Their effect on the kinetics of chemiluminescent reactions has not been systematically studied to date. It seems appropriate to study the model chemiluminescent reaction in typical organized molecular systems (micellar), which can be considered as the model of biological membranes. The micellar medium enables studying the mechanism of chemiluminescent reactions due to the effect of the micropreconcentration and separation of the substrate and intermediate products. It also helps to control the chemiluminescent reactions, primarily, to increase their sensitivity and expand the range of applicability, for example, to lower the matrix effect or combine components with limited mutual solubility. The effect of organized molecular (in particular, micellar) systems on chemiluminescent reactions has not been systematically studied; however, there are publications where such an effect has been considered. Various chemiluminescent reactions in organized molecular systems were studied [7–17] to increase the quantum yield of the chemiluminescence and improve the sensitivity of the chemiluminescent analytical techniques. The use of surfactants in the lucigenin chemiluminescent reactions improved the methods for determining vitamins C and P [7], nicotine [8], estrogen [9], cholesterol [10], and the platelet-activating factor [11] by increasing the quantum yield of the che-
miluminescence. In some studies, the transition to a micellar medium changed the spectral characteristics of the chemiluminescence. For example, in addition to increasing the quantum yield of the process, the appearance of radiation from the primary emitter was observed in the reaction of 10,10'-dimethyl-9,9'biacridinium nitrate with alkali and hydrogen peroxide, performed in a micel
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