Circulating levels of PTEN and KLLN in papillary thyroid carcinoma: can they be considered as novel diagnostic biomarker
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ORIGINAL ARTICLE
Circulating levels of PTEN and KLLN in papillary thyroid carcinoma: can they be considered as novel diagnostic biomarkers? S. Adeleh Razavi1 Mohammad Hossein Modarressi1,2 Parichehr Yaghmaei1 S. Mohammad Tavangar3 Mehdi Hedayati4 ●
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Received: 19 April 2017 / Accepted: 27 June 2017 © Springer Science+Business Media, LLC 2017
Abstract Purpose PTEN and KLLN are two tumor suppressor genes located in 10q23, share a bidirectional promoter and have roles in carcinogenesis. Formerly, the role of PTEN mutations and KLLN epimutations were identified in incidence of thyroid lesions in individuals with Cowden syndrome, a rare autosomal dominant inherited disorder. This study is the first of its type to assess PTEN and KLLN circulating levels in patients with sporadic papillary thyroid carcinoma (PTC) and compare to patients with multinodular goiter (MNG) and healthy individuals. Methods Plasma levels of PTEN and KLLN were determined by enzyme-linked immunosorbent assay in three groups consisted of PTC (n = 33), MNG (n = 26) and healthy persons (n = 30). The association of demographic/ pathological characteristics with the levels of PTEN and KLLN were evaluated. Results A significant lower plasma levels of PTEN and KLLN were observed in PTC patients compared with those of healthy persons (PTEN, 9.43 ± 3.20 vs. 16.96 ± 1.28 ng/ml, P = 0.000; KLLN, 1.81 ± 0.83 vs. 2.57 ± 1.09 ng/ml,
* Mehdi Hedayati [email protected] [email protected] 1
Department of Biology, Faculty of Basic Sciences, Science and Research Branch, Islamic Azad University, Tehran, Iran
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Department of Medical Genetics, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran
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Department of Pathology, Shariati Hospital, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran
4
Cellular and Molecular Endocrine Research Center, Research Institute for Endocrine Sciences, Shahid Beheshti University of Medical Sciences, Tehran, Iran
P = 0.005), while no statistical difference was found between PTC and MNG groups. Patients with MNG lesion had significantly lower levels of PTEN/KLLN (PTEN, 9.62 ± 2.97 vs. 16.96 ± 1.28 ng/ml, P = 0.000; KLLN, 1.34 ± 0.86 vs. 2.57 ± 1.09 ng/ml, P = 0.000) compared to the healthy controls. The demographic/pathological characteristics did not demonstrate an association with the levels of PTEN and KLLN. Conclusions The study suggests that the lowered levels of PTEN and KLLN are associated with both sporadic PTC and MNG tumorigenesis, but they cannot be considered as circulating biomarkers for differential diagnosis between malignancy and benignity in indeterminate thyroid nodules. Keywords Papillary thyroid carcinoma PTEN protein KLLN protein Biomarker Enzyme-Linked Immunosorbent Assay ●
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Abbreviations CS Cowden syndrome CSL Cowden syndrome-like ELISA enzyme-linked immunosorbent assay FNAB fine needle aspiration biopsy MNG multinodular goiter PTC papillary thyroid carcinoma TC thyroid cancer SD standard deviation
Introduction Similar to worldwide papillary thyr
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