Combination Therapy with Curcumin Alone Plus Piperine Ameliorates Ovalbumin-Induced Chronic Asthma in Mice
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ORIGINAL ARTICLE
Combination Therapy with Curcumin Alone Plus Piperine Ameliorates Ovalbumin-Induced Chronic Asthma in Mice Preeti S. Chauhan,1 Anju Jaiswal,1 Subhashini,1 and Rashmi Singh1,2
Abstract— Allergic asthma is an inflammatory condition accompanied by inflammation as well
as oxidative stress. Supplementation of an anti-inflammatory agent having antioxidant properties may have therapeutic effects against this disease. Over the recent decades, the interest in combination therapy as new alternative medication has increased and it offers numerous benefits along with noticeable lack of toxicity as well as side effects. In this study, protective effects of curcumin alone and in combination with piperine were evaluated in mouse model of allergic asthma. Balb/c mice were sensitized on days 0, 7, and 14 and challenged from days 16–30 on alternate days with ovalbumin (OVA). Mice were pretreated with curcumin (Cur; 10 and 20 mg/kg) and piperine (Pip; 5 mg/kg) alone and in combination via the intraperitoneal route on days 16–30 and compared with intranasal curcumin (5 mg/kg) treatment. Blood, bronchoalveolar lavage fluid (BALF), and lungs were collected after mice were sacrificed on day 31st. Mice immunized with OVA have shown significant increase in airway inflammation and oxidative stress as determined by oxidative stress markers. A significant suppression was observed with all the treatments, but intranasal curcumin treatment group has shown maximum suppression. So, among all the treatment strategies utilized, intranasal curcumin administration was most appropriate in reducing inflammation and oxidative stress and possesses therapeutic potential against allergic asthma. Present study may prove the possibility of development of curcumin nasal drops towards treatment of allergic asthma. KEY WORDS: inflammation; oxidative stress; ovalbumin; curcumin; piperine; asthma.
INTRODUCTION Asthma is emerging as one of the most common chronic respiratory disease affecting a large human population. World Health Organization (WHO) estimated about 300 million people suffering from asthma and over 80% of asthma deaths are reported from low and lower-middle income countries creating a burden on society [1]. This 1
Department of Zoology, MMV, Banaras Hindu University, Varanasi, 221005, India 2 To whom correspondence should be addressed at Department of Zoology, MMV, Banaras Hindu University, Varanasi, 221005, India. E-mail: [email protected]
disease is characterized by a major condition of inflammation predominantly in the airways. There is increasing evidence that inflammation is main characteristic of asthma, which results in increased oxidative stress in the airways [2]. Inflammatory cells in the airways such as macrophages, neutrophils, and eosinophils release increased amounts of reactive oxygen species in asthmatic patients [3–5]. The increased release of reactive oxygen species can result in direct oxidative damage to epithelial cells and lead to cell shedding [6]. Studies have suggested that the preventive trea
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