Companion animals get close to the toxic aspects of antropogenic world: cytotoxicity of phthalates and bisphenol A on do
- PDF / 1,692,239 Bytes
- 10 Pages / 547.087 x 737.008 pts Page_size
- 99 Downloads / 139 Views
(0123456789().,-volV) ( 01234567 89().,-volV)
ORIGINAL ARTICLE
Companion animals get close to the toxic aspects of antropogenic world: cytotoxicity of phthalates and bisphenol A on dog testicular primary cells Koray Tekin . Pinar Arslan . Beste Cil . Ayhan Filazi . Ergun Akc¸ay . Begum Yurdakok-Dikmen
Received: 4 December 2019 / Accepted: 11 May 2020 Springer Nature B.V. 2020
Abstract Phthalates, which are among the most abundant plasticizers, have detrimental effects on the reproductive system. Similar to human, dogs are prominently exposed to phthalates in daily routines at low concentrations; while toys, training devices and commercial dog foods are considered as the primary sources of exposure. This study aimed to reveal and compare the cytotoxic effects of selected phthalates (Benzyl butyl phthalate (BBP), Diethyl phthalate (DEP), Bis (2-ethylhexyl) phthalate (DEHP), Di‘isobutyl’ phthalate (DIBP), Di-‘isodecyl’ phthalate (-DIDP) Di-‘isononyl’ phthalate (DINP), Dimethyl phthalate (DMP), Di-n-octyl phthalate (DNOP)), and Bisphenol A (BPA) following 24 h exposure on primary testicular parenchymal cells of dog in vitro at concentrations between 0.001 and 2.5 nM. According to cytotoxicity results, DEHP was found to be the
most toxic phthalate with IC50 at 22.53 lM; while DMP was the least (169.17 nM). IC50 of BPA was 161.81 nM, less than the average (61.95 nM) of phthalates. In addition, dog primary testicular cells were found more susceptible to the high molecular weight phthalates (DNOP, DEHP, DINP, DIDP) than low molecular weight phthalates (DMP, DEP, DIBP, BBP). Further studies should focus on morphological, physiological and molecular differences to comprehend the mechanisms involved as well as decreasing the risk for impaired spermatogenesis caused by environmental toxicants in companion animal medicine.
K. Tekin B. Cil E. Akc¸ay Department of Reproduction and Artificial Insemination, Faculty of Veterinary Medicine, Ankara University, Diskapi, 06110 Ankara, Turkey
Highlight
P. Arslan Department of Biology, Faculty of Science, Ankara University, Tandogan, 06100 Ankara, Turkey A. Filazi B. Yurdakok-Dikmen (&) Department of Pharmacology and Toxicology, Faculty of Veterinary Medicine, Ankara University, Diskapi, 06110 Ankara, Turkey e-mail: [email protected]; [email protected]
Keywords Bisphenol A Phthalates Canine testicular cells In vitro toxicity
• Human and dog share similar environment where pollutants such as plasticizers lead reproductive disorders in both species. • DEHP was the most toxic on primary testicular cell culture of dog. • Comparative evaluation of endocrine disrupters on male reproductive system would allow us to understand the protective mechanisms involved.
123
Cytotechnology
Introduction Phthalates and Bisphenol A (BPA) are the most common synthetic chemicals, widely used in automotive, personal care consumer product and medical industries. Phthalates, also called plasticizers, are a group of chemical substances used to soften plastics. Some pht
Data Loading...
