Comparative analysis of basidiomycetous laccase genes in forest soils reveals differences at the cDNA and DNA levels
- PDF / 426,945 Bytes
- 11 Pages / 547.087 x 737.008 pts Page_size
- 41 Downloads / 162 Views
REGULAR ARTICLE
Comparative analysis of basidiomycetous laccase genes in forest soils reveals differences at the cDNA and DNA levels Xiangbi Chen & Yirong Su & Xunyang He & Yueming Liang & Jinshui Wu
Received: 14 October 2011 / Accepted: 22 August 2012 / Published online: 5 September 2012 # Springer Science+Business Media B.V. 2012
Abstract Aims This study was undertaken to investigate laccasecontaining basidiomycete communities at the cDNA and DNA levels and to assess the influences of vegetation and soil types on the basidiomycete communities in forest soils. Materials and methods Soil samples were collected from the upper soil layers of two typical subtropical forests (a broad-leaved forest developed in Karst Responsible Editor: Katharina Pawlowski. Electronic supplementary material The online version of this article (doi:10.1007/s11104-012-1440-z) contains supplementary material, which is available to authorized users. X. Chen : Y. Su : X. He : Y. Liang : J. Wu Key Laboratory of Agro-ecological Processes in Subtropical Region, Institute of Subtropical Agriculture, the Chinese Academy of Sciences, Changsha 410125, China X. Chen : Y. Su : X. He : Y. Liang Huanjiang Observation and Research Station for Karst Eco-systems, Huanjiang 547100, China X. Chen Graduate School of Chinese Academy of Sciences, Beijing 100039, China Y. Su (*) Institute of Subtropical Agriculture, the Chinese Academy of Sciences, Changsha, Hunan 410125, China e-mail: [email protected]
limestone soil and an artificial coniferous forest with Pinus massoniana in red soil) in China. The basidiomycete communities were characterized by cloning and sequencing of the laccase genes at both the cDNA and DNA levels. Compositions of lignin monomers were determined by gas chromatography–mass spectrometry. Results Most of the laccase genes obtained in this study were new, highlighting the research gap of this functional group. The trends of the basidiomycetous laccase gene diversity among the upper soil layers of the two forests were consistent between the cDNA and DNA levels. The Agaricales had high activity because they dominated all the tested soils. However, the total basidiomycete communities reflected at the cDNA and DNA levels were significantly different due to the presence of some quiescent basidiomycetous groups. Almost all of the lignin components were decomposed from the O to the A layers in the two forests, and laccases produced by Agaricales were likely responsible for the decomposition of guaiacyl monomers. Both vegetation and soil types had great influences on the active laccasecontaining basidiomycete communities, primarily via the pH, C/N, and the contents of lignin monomers. Conclusions The cDNA- and DNA-level approaches presented good consistency of diversities but different compositions of laccase-containing basidiomycete communities, thus emphasizing the importance of focusing on laccase genes at the cDNA level in future studies. It is the quality but not the quantity of SOM to determine the diversity and composition of the active lacca
Data Loading...
