Comparison of RCAS1 and metallothionein expression and the presence and activity of immune cells in human ovarian and ab
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Comparison of RCAS1 and metallothionein expression and the presence and activity of immune cells in human ovarian and abdominal wall endometriomas Lukasz Wicherek*1, Magdalena Dutsch-Wicherek2, Krystyna Galazka3, Tomasz Banas2, Tadeusz Popiela4, Agata Lazar1 and Beata KleinrokPodsiadlo4 Address: 1Department of Gynecology and Infertility of the Jagiellonian University, 23 Kopernik Str, 31-501 Krakow, Poland, 2Department of Pathomorphology of the Jagiellonian University, 17 Grzegorzecka Str, 31-531 Krakow, Poland, 3ENT Department of the Jagiellonian University, 2 Sniadeckich Str, 31-531 Krakow, Poland and 4Department of the General Surgery of the Jagiellonian University, 40 Kopernik Str, 31-501 Krakow, Poland Email: Lukasz Wicherek* - [email protected]; Magdalena Dutsch-Wicherek - [email protected]; Krystyna Galazka - [email protected]; Tomasz Banas - [email protected]; Tadeusz Popiela - [email protected]; Agata Lazar - [email protected]; Beata KleinrokPodsiadlo - [email protected] * Corresponding author
Published: 14 August 2006 Reproductive Biology and Endocrinology 2006, 4:41
doi:10.1186/1477-7827-4-41
Received: 17 June 2006 Accepted: 14 August 2006
This article is available from: http://www.rbej.com/content/4/1/41 © 2006 Wicherek et al; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract Background: The coexistence of endometrial and immune cells during decidualization is preserved by the ability of endometrial cells to regulate the cytotoxic immune activity and their capability to be resistant to immune-mediated apoptosis. These phenomena enable the survival of endometrial ectopic cells. RCAS1 is responsible for regulation of cytotoxic activity. Metallothionein expression seems to protect endometrial cells against apoptosis. The aim of the present study was to evaluate RCAS1 and metallothionein expression in human ovarian and scar endometriomas in relation to the presence of immune cells and their activity. Methods: Metallothionein, RCAS1, CD25, CD69, CD56, CD16, CD68 antigen expression was assessed by immunohistochemistry in ovarian and scar endometriomas tissue samples which were obtained from 33 patients. The secretory endometrium was used as a control group (15 patients). Results: The lowest metallothionein expression was revealed in ovarian endometriomas in comparison to scar endometriomas and to the control group. RCAS1 expression was at the highest level in the secretory endometrium and it was at comparable levels in ovarian and scar endometriomas. Similarly, the number of CD56-positive cells was lower in scar and ovarian endometriomas than in the secretory endometrium. The highest number of macrophages was found in ovarian endometriomas. RCAS1-positive macrophages were observed only in ovarian endometr
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