Computational Design of Ligand Binding Proteins

This volume provides a collection of protocols and approaches for the creation of novel ligand binding proteins, compiled and described by many of today's leaders in the field of protein engineering. Chapters focus on modeling protein ligand binding

  • PDF / 15,179,666 Bytes
  • 379 Pages / 504.63 x 737.01 pts Page_size
  • 13 Downloads / 216 Views

DOWNLOAD

REPORT


Barry L. Stoddard Editor

Computational Design of Ligand Binding Proteins

METHODS

IN

MOLECULAR BIOLOGY

Series Editor John M. Walker School of Life and Medical Sciences University of Hertfordshire Hatfield, Hertfordshire, AL10 9AB, UK

For further volumes: http://www.springer.com/series/7651

Computational Design of Ligand Binding Proteins Edited by

Barry L. Stoddard Division of Basic Sciences, Fred Hutchinson Cancer Research Center, Seattle, Washington, USA

Editor Barry L. Stoddard Division of Basic Sciences Fred Hutchinson Cancer Research Center Seattle, Washington, USA

ISSN 1064-3745 ISSN 1940-6029 (electronic) Methods in Molecular Biology ISBN 978-1-4939-3567-3 ISBN 978-1-4939-3569-7 (eBook) DOI 10.1007/978-1-4939-3569-7 Library of Congress Control Number: 2016937968 © Springer Science+Business Media New York 2016 This work is subject to copyright. All rights are reserved by the Publisher, whether the whole or part of the material is concerned, specifically the rights of translation, reprinting, reuse of illustrations, recitation, broadcasting, reproduction on microfilms or in any other physical way, and transmission or information storage and retrieval, electronic adaptation, computer software, or by similar or dissimilar methodology now known or hereafter developed. The use of general descriptive names, registered names, trademarks, service marks, etc. in this publication does not imply, even in the absence of a specific statement, that such names are exempt from the relevant protective laws and regulations and therefore free for general use. The publisher, the authors and the editors are safe to assume that the advice and information in this book are believed to be true and accurate at the date of publication. Neither the publisher nor the authors or the editors give a warranty, express or implied, with respect to the material contained herein or for any errors or omissions that may have been made. Printed on acid-free paper This Humana Press imprint is published by Springer Nature The registered company is Springer Science+Business Media LLC New York

Preface Introduction: Design and Creation of Ligand-Binding Proteins The appropriate balance of ligand binding affinity and specificity is a fundamental feature of most if not all biological processes, including immune recognition, cellular metabolism, regulation of gene expression, and cell signaling. The ability to accurately predict and recapitulate the physical basis for ligand binding behavior is therefore a crucial part of understanding and manipulating such biological phenomena. It also represents a critical technical requirement in the reciprocal fields of drug design and protein engineering. This book provides a collection of protocols and approaches, compiled and described by many of today’s leaders in the field of protein engineering, that they apply to the problem of creating ligand-binding proteins that display desirable combinations of target affinity and specificity. The descriptions provided by each chapter’s authors also provide a sn