Creation of fertility-restored materials for Ogura CMS in Brassica oleracea by introducing Rfo gene from Brassica napus
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ORIGINAL ARTICLE
Creation of fertility‑restored materials for Ogura CMS in Brassica oleracea by introducing Rfo gene from Brassica napus via an allotriploid strategy Hai‑long Yu1 · Zhi‑yuan Li1 · Wen‑jing Ren1 · Feng‑qing Han1 · Li‑mei Yang1 · Mu Zhuang1 · Hong‑hao Lv1 · Yu‑mei Liu1 · Zhi‑yuan Fang1 · Yang‑yong Zhang1 Received: 28 May 2020 / Accepted: 9 June 2020 © The Author(s) 2020
Abstract Key message Ogura CMS fertility-restored materials, with 18 chromosomes, normal seed setting, stable fertility and closer genetic background to the parent Chinese kale, were successfully developed in B. oleracea via a triploid strategy for the first time. Abstract Ogura cytoplasmic male sterility (CMS) is the most widely used sterile type in seed production for commercial hybrids of Brassica oleracea vegetables. However, the natural Ogura CMS restorer line has not been found in B. oleracea crops. In this study, the triploid strategy was used with the aim to create euploid B. oleracea progenies with the Rfo gene. The allotriploid AAC hybrid YL2 was used as a male parent to backcross with Ogura CMS Chinese kale. After successive backcrosses, the BC2 Rfo-positive individual 16CMSF2-11 and its BC3 progenies, with 18 chromosomes, were developed, which were morphologically identical to the parent Chinese kale. Compared with F 1 and B C1 plants, it showed stable fertility performance, and regular meiosis behavior and could produce seeds normally under natural pollination. The genomic composition analysis of Rfo-positive progenies by using molecular markers showed that more than 87% of the C-genome components of B C3 Rfo-progenies recovered to the parent Chinese kale, while most or all of the An-genome segments were lost in 16CMSF2-11 and its progenies. The results suggested that the genetic background of Rfo-positive individuals was closer to that of the parent Chinese kale along with backcrossing. Hereof, the Ogura CMS fertility-restored materials of Chinese kale were successfully created via triploid strategy for the first time, providing a bridge for utilizing the Ogura CMS B. oleracea germplasm in the future. Moreover, our study indicates that the triploid strategy is effective for transferring genes from B. napus into B. oleracea.
Introduction Communicated by Carlos F. Quiros. Hai-long Yu, Zhi-yuan Li, and Wen-jing Ren have contributed equally to this study. Electronic supplementary material The online version of this article (https://doi.org/10.1007/s00122-020-03635-8) contains supplementary material, which is available to authorized users. * Zhi‑yuan Fang [email protected] * Yang‑yong Zhang [email protected] 1
Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, No. 12 ZhongGuanCun South St., Beijing 100081, People’s Republic of China
Brassica oleracea is a typical cross-pollination species, and its F1 hybrids exhibit strong heterosis, such as the high yield, better quality, increased uniformity, wide adaptability and resistance to biotic stresses in single cross-hybrids
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