Crosstalk-free colloidosomes for high throughput single-molecule protein analysis
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tps://doi.org/10.1007/s11426-020-9818-9
SPECIAL ISSUE: 2020 Emerging Investigator Issue
Crosstalk-free colloidosomes for high throughput single-molecule protein analysis 1
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Kun Yin , Xi Zeng , Xuan Liang , Haopai Wei , Huimin Zeng , Wei Qi , Weidong Ruan , 1 1,2 1* Yanling Song , Chaoyong Yang & Zhi Zhu 1
The Key Laboratory of Chemical Biology of Fujian Province, the MOE Key Laboratory of Spectrochemical Analysis & Instrumentation, State
Key Laboratory of Physical Chemistry of Solid Surfaces, Collaborative Innovation Center of Chemistry for Energy Materials, Department of Chemical Engineering, Department of Chemical Biology, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen 361005, China; 2 Institute of Molecular Medicine, Renji Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200127, China Received June 7, 2020; accepted July 7, 2020; published online August 18, 2020
Droplet microfluidics is a powerful platform for high-throughput single-molecule protein analysis. However, the issues of coalescence and crosstalk of droplets compromise the accuracy of detection and hinder its wide application. To address these limitations, a novel colloidosome-based method was presented by combining a Pickering emulsion with droplet microfluidics for single-molecule protein analysis. Utilizing the self-assembly of easily synthesized colloidal surfactant F-SiO2 NPs at the water/ oil interface, the colloidosomes are rigidly stabilized and can effectively avoid the leakage of fluorescent molecules. The crosstalk-free colloidosomes enable high-throughput single-molecule protein analysis, including heterogenous dynamic studies and digital detection. As a robust and accurate method, colloidosome-based microfluidics is promising as a powerful tool for a wide variety of applications, such as directed enzyme evolution, digital enzyme-linked immunosorbent assay (ELISA), and screening of antibiotics. colloidosomes, droplet microfluidics, crosstalk-free, single-molecule analysis, protein detection Citation:
Yin K, Zeng X, Liang X, Wei H, Zeng H, Qi W, Ruan W, Song Y, Yang C, Zhu Z. Crosstalk-free colloidosomes for high throughput single-molecule protein analysis. Sci China Chem, 2020, 63, https://doi.org/10.1007/s11426-020-9818-9
1 Introduction Due to the critical role of proteins in life processes, the exploration of the biological reactions at the single-molecule level would enable study of their heterogeneity instead of their average behavior in bulk measurements and provide a sensitive and high-resolution method for the detection of low abundance proteins in complex matrices [1–7]. Notably, none of the single-molecule reaction can be characterized without the assistance of a confined space [8,9]. Droplet microfluidics is becoming a powerful tool for single-
molecule protein analysis, because of its capability of compartmentalizing biological reactions at the single-molecule level in thousands or even millions of spatially separated uniform microreactors [10–15]. In droplet
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