Cypermethrin alters the status of oxidative stress in the peripheral blood: relevance to Parkinsonism
- PDF / 6,037,917 Bytes
- 10 Pages / 547.087 x 737.008 pts Page_size
- 50 Downloads / 181 Views
ORIGINAL PAPER
Cypermethrin alters the status of oxidative stress in the peripheral blood: relevance to Parkinsonism Pratibha Tripathi & Ashish Singh & Sonal Agrawal & Om Prakash & Mahendra Pratap Singh
Received: 24 April 2014 / Accepted: 17 September 2014 / Published online: 1 October 2014 # University of Navarra 2014
Abstract Parkinson’s disease (PD) is a motor scarcity disorder characterized by the striatal dopamine deficiency owing to the selective degeneration of the nigrostriatal dopaminergic neurons. While oxidative stress is implicated in PD, prolonged exposure to moderate dose of cypermethrin induces Parkinsonism. The study aimed to investigate the status of oxidative stress indicators and antioxidant defence system of the polymorphonuclear leukocytes (PMNs), platelets and plasma to delineate the effect of Parkinsonian dose of cypermethrin in the peripheral blood of rats and its subsequent relevance to Parkinsonism. Nitrite content, lipid peroxidation (LPO) and activity of superoxide dismutase (SOD), catalase, glutathione reductase (GR) and glutathione-S-transferase (GST) were measured in the PMNs, platelets and plasma of control and cypermethrin-treated rats in the presence or absence of a microglial activation inhibitor, minocycline or a dopamine precursor containing the peripheral 3,4-dihydroxyphenylalanine decarboxylase inhibitor, named syndopa, employing the standard procedures. The striatal dopamine was measured to assess the degree of neurodegeneration/neuroprotection. Cypermethrin increased nitrite and LPO in the plasma, platelets and PMNs while it reduced the striatal dopamine P. Tripathi : A. Singh : S. Agrawal : M. P. Singh (*) CSIR-Indian Institute of Toxicology Research, Mahatma Gandhi Marg, Post Box No. 80, Lucknow 226 001, Uttar Pradesh, India e-mail: [email protected] O. Prakash Banaras Hindu University, Varanasi 221 005, Uttar Pradesh, India
content. Catalase and GST activity were increased in the PMNs and platelets; however, it was reduced in the plasma. Conversely, SOD and GR activities were reduced in the PMNs and platelets but increased in the plasma. Minocycline or syndopa reduced the cypermethrinmediated changes towards normalcy. The results demonstrate that cypermethrin alters the status of oxidative stress indicators and impairs antioxidant defence system of the peripheral blood, which could be effectively salvaged by minocycline or syndopa. The results could be of value for predicting the nigrostriatal toxicity relevant to Parkinsonism. Keywords Parkinsonism . Peripheral blood . Cypermethrin . Minocycline . Syndopa
Introduction Parkinson’s disease (PD) is a chronic and idiopathic neurodegenerative disorder characterized by the loss of dopaminergic neurons of the nigrostriatal pathway, which results in the postural instability, bradykinesia, resting tremor and muscular rigidity [8, 35]. Despite sporadic nature, ageing, genetic predisposition and environmental exposure to pesticides and metals are implicated in PD pathogenesis [8, 31, 35]. Epidemiological investi
Data Loading...
