Date palm virus A: first plant virus found in date palm trees
- PDF / 771,859 Bytes
- 4 Pages / 595.276 x 790.866 pts Page_size
- 79 Downloads / 222 Views
SHORT REPORT
Date palm virus A: first plant virus found in date palm trees Ayoub Maachi1 · Tatsuya Nagata2 · João Marcos Fagundes Silva2 Received: 20 July 2020 / Accepted: 26 September 2020 © Springer Science+Business Media, LLC, part of Springer Nature 2020
Abstract In this work, a novel ssRNA (+) viral genomic sequence with gene organization typical of members of the subfamily Quinvirinae (family Betaflexiviridae) was identified using high- throughput sequencing data of date palm obtained from the Sequence Read Archive database. The viral genome sequence consists of 7860 nucleotides and contains five ORFs encoding for the replication protein (Rep), triple gene block proteins 1, 2, 3 (TGB 1, 2, and 3), and coat protein (CP). Phylogenetic analysis based on the Rep and the CP amino acid sequences showed the closest relationship to garlic yellow mosaic-associated virus (GYMaV). Based on the demarcation criteria of the family Betaflexiviridae, this new virus, provisionally named date palm virus A (DPVA), could constitute a member of a novel genus. However, considering that DPVA and GYMaV share the same genomic organization and that they cluster together on the Rep phylogenetic analysis, they could also constitute a novel genus together, highlighting the necessity of a revision of the taxonomic criteria of the family Betaflexiviridae. Keywords Date palm virus A · Betaflexiviridae · High-throughput sequencing · New genus · New species Virus members of the family Betaflexiviridae have flexuous filament virions and ssRNA (+) genome, ranging from 5.9 to 9.0 kb. The genomic RNA is presumably capped at the 5′ end and has a polyadenylated tail at the 3′ end [1]. Betaflexiviruses were reported from a diverse host range of plant species, from vegetable crops to fruit trees [1]. Date palm tree, a crop intensively cultivated and of major economic importance in Northern African and Middle East countries [2], is very robust and, until now, no virus whatsoever was reported to infect this plant species. Only African oil palm ringspot virus of this family was reported to infect palm trees (family: Arecaceae) [3, 4]; however, no reports were found about the occurrence of this virus in palm tree. Here, a plant virus sequence was found in high-throughput sequencing (HTS) data of date palm trees (Phoenix dactylifera) from the Sequence Read Archive (SRA) public database. Thus, we report the characteristics of the genomic sequence of a novel Edited by Karel Petrzik. * João Marcos Fagundes Silva [email protected] 1
R&D Department, Abiopep S.L, Espinardo 30100, Murcia, Spain
Department of Cell Biology, Institute of Biological Sciences, University of Brasília (UnB), Brasília, Federal District 70910‑900, Brazil
2
virus belonging to the family Betaflexiviridae, tentatively named date palm virus A (DPVA). Raw transcriptomic data from SRA under the NCBI BioProject number PRJNA472694, used for comparison of gene expression in male and female date palm flowers, were trimmed with BBDuk v38.08 to remove adapters and lowqualit
Data Loading...
