Degradation of Influenza Virus Nucleoprotein by N 2 Gas Plasma
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Degradation of Influenza Virus Nucleoprotein by N2 Gas Plasma Sakudo A1, Hayashi N2, Shimizu N3, Imanishi Y3, Shintani H4 1 Laboratory of Biometabolic Chemistry, School of Health Sciences, University of the Ryukyus, Nishihara, Okinawa 903-0215, Japan. 2 Department of Electrical and Electronic Engineering, Faculty of Science and Engineering, Saga University, Saga-city, Saga, 840-8502, Japan. 3 NGK Insulators, Mizuho-ku, Nagoya 467-8530, Japan. 4 Faculty of Science and Engineering, Chuo University, Bunkyo-ku, Tokyo 112-8551, Japan. ABSTRACT Using a N2 gas plasma apparatus (BLP-TES, NGK Insulators, Ltd), we showed that N2 gas plasma treatment of influenza A virus caused degradation of viral nucleoproteins. These findings suggest that N2 gas plasma treatment may contribute to infection prevention control for influenza. INTRODUCTION To date, N2 gas plasma has been successfully produced by giving a short-time high-voltage pulse to N2, an inert gas, using a static induction (SI) thyristor power supply; and a result that spore forming bacteria and endotoxin, known to be difficult to inactivate, can be inactivated by the N2 gas plasma has been obtained [1]. Infection mediated by medical devices is a major contributor to hospital-acquired infection; however, it is questioned that medical devices and instruments are not durable against treatment with existing sterilization methods including autoclaved sterilization. Recently, hydrogen peroxide gas plasma sterilization, which is applicable to this type of equipment, was proposed, but possible risks such as toxic residue (hydrogen peroxide) was being pointed out [2]. In addition, it was reported that the hydrogen peroxide gas plasma sterilization was not effective to endotoxin, although residual endotoxin derived from bacteria may cause symptoms including fever [3]. Under such circumstances, this study aims to expansively develop nano-interface plasma study in the virology area by reacting N2 gas plasma generated by the above method with nano particle virus based on previously obtained research products. In this study, we treated influenza virus, an enveloped virus, with plasma and analyzed the effect on sterilizing efficiency and viral proteins by studying biochemical changes to the nucleoprotein induced by the plasma treatment. Our investigation acts as a foundation study for the application of this technology for sterilization and disinfection.
EXPERIMENT Interaction between influenza virus, an enveloped virus, and N2 gas plasma was analyzed. The size of influenza virus is about 80 to 120 nm in diameter, and the virus has a lipid membrane called an envelope. The envelope has protein called hemagglutinin, which is a protein required for absorption into a cell, and neuraminidase, which is a protein required for release of viruses from the cell. Moreover, RNA entangled with a protein called nucleoprotein exists in the central part of the virus. In this study, we analyzed changes to the nucleoprotein of influenza virus after N2 gas plasma treatment. BLP-TES (NGK Insulators, Ltd
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