Degradation of Sesame Oil Phenolics Using Magnetic Immobilized Laccase
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Degradation of Sesame Oil Phenolics Using Magnetic Immobilized Laccase Reza Amin1 · Alireza Khorshidi1 · Wolfgang Bensch2 · Svenja Senkale2 · Mohammad Ali Faramarzi3 Received: 24 December 2019 / Accepted: 19 April 2020 © Springer Science+Business Media, LLC, part of Springer Nature 2020
Abstract Phenolic compounds exhibit toxic effects and there are still many challenges to find an efficient way for degradation and removal of phenol especially from food. In the present study, magnetically immobilized laccase was prepared and applied as an efficient heterogeneous biocatalyst for biodegradation of phenol from sesame oil. Laccase was attached covalently to magnetic Fe3O4 nanospheres and nanorods, and the characteristics of the immobilized enzyme were studied. The magnetic supports were analyzed by scanning electron microscopy, X-ray diffraction, vibrating sample magnetometry, and Fouriertransform infrared spectroscopy. Storage stability analysis of immobilized enzyme showed that more than 70% of initial activity was kept after 15 days at 4 °C. More than 70% phenol degradation was achieved and a 60% decrease in the activity of immobilized laccase was observed after 20 independent cycles. The development of enzyme immobilization techniques on magnetic supports may expand the potential applications of heterogeneous biocatalysts in food industries. Graphic Abstract
Keywords Laccase · Immobilization · Sesame oil · Magnetite · Phenol · Degradation
* Alireza Khorshidi [email protected] * Mohammad Ali Faramarzi [email protected] 1
Department of Chemistry, Faculty of Sciences, University of Guilan, P.O. Box 41335‑1914, Rasht, Guilan, Iran
2
Institute of Inorganic Chemistry, Kiel University, Max‑Eyth Straβe 2, 24118 Kiel, Germany
3
Department of Pharmaceutical Biotechnology, Faculty of Pharmacy, Tehran University of Medical Sciences, P.O. Box 14155–6451, Tehran 1417614411, Iran
Abbreviations ABTS 2,2′-Azino-bis 3-ethylbenzthiazoline-6-sulfonic acid is a peroxidase substrate suitable for enzyme activity measurement. APTES 3-Triethoxysilylpropylamine was used for amination FT-IR Fourier-transform infrared IU The enzyme international unit, One U is defined as the amount of the enzyme that transforms one µmole of substrate / minute.
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PAMAM Poly(amidoamine) is a type of dendrimer containing repetitively branched subunits of amide and amine moieties that is commonly used for surface modification. PEG Polyethylene glycol TEOS Tetraethyl orthosilicate VSM Vibrating sample magnetometer, was used to determine magnetization properties of nanostructured iron oxide supports. XRD X-ray diffraction, was used to study the structure, composition, and physical properties of synthesized MNPs. SEM Scanning electron microscope was used as a sample imaging tool
1 Introduction Polyphenolic compounds that exhibit severe toxicity to human health are commonly found in many foods such as seeds, nuts, fruits, and beans [1]. To date, conventional methods for treatment of industrial effluen
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